Zhu Liang, Hou Qian, Yan Meijun, Gao Wentao, Tang Guoke, Liu Zhiqing
Department of Orthopedics, Zhuzhou Hospital Affiliated to Xiangya School of Medicine, Central South University, Zhuzhou 412001, China.
Department of Orthopedics, Shanghai General Hospital, Shanghai Jiaotong University, Shanghai 200080, China.
Pharmaceutics. 2023 Sep 26;15(10):2384. doi: 10.3390/pharmaceutics15102384.
Osteogenic scaffolds reproducing the natural bone composition, structures, and properties have represented the possible frontier of artificially orthopedic implants with the great potential to revolutionize surgical strategies against the bone-related diseases. However, it is difficult to achieve an all-in-one formula with the simultaneous requirement of favorable biocompatibility, flexible adhesion, high mechanical strength, and osteogenic effects. Here in this work, an osteogenic hydrogel scaffold fabricated by inorganic-in-organic integration between amine-modified bioactive glass (ABG) nanoparticles and poly(ethylene glycol) succinimidyl glutarate-polyethyleneimine (TSG-PEI) network was introduced as an all-in-one tool to flexibly adhere onto the defective tissue and subsequently accelerate the bone formation. Since the N-hydroxysuccinimide (NHS)-ester of tetra-PEG-SG polymer could quickly react with the NH-abundant polyethyleneimine (PEI) polymer and ABG moieties, the TSG-PEI@ABG hydrogel was rapidly formed with tailorable structures and properties. Relying on the dense integration between the TSG-PEI network and ABG moieties on a nano-scale level, this hydrogel expressed powerful adhesion to tissue as well as durable stability for the engineered scaffolds. Therefore, its self-endowed biocompatibility, high adhesive strength, compressive modulus, and osteogenic potency enabled the prominent capacities on modulation of bone marrow mesenchymal stem cell (BMSCs) proliferation and differentiation, which may propose a potential strategy on the simultaneous scaffold fixation and bone regeneration promotion for the tissue engineering fields.
能够重现天然骨成分、结构和特性的成骨支架代表了人工骨科植入物的前沿领域，具有彻底改变针对骨相关疾病手术策略的巨大潜力。然而，要实现一种同时具备良好生物相容性、灵活粘附性、高机械强度和成骨效果的一体化配方并非易事。在本研究中，一种通过胺改性生物活性玻璃（ABG）纳米颗粒与聚（乙二醇）琥珀酰亚胺戊二酸酯 - 聚乙烯亚胺（TSG - PEI）网络之间的无机 - 无机整合制备的成骨水凝胶支架被引入，作为一种一体化工具，可灵活粘附于缺损组织并随后加速骨形成。由于四聚乙二醇 - 琥珀酰亚胺戊二酸酯（tetra - PEG - SG）聚合物的N - 羟基琥珀酰亚胺（NHS）酯能迅速与富含NH的聚乙烯亚胺（PEI）聚合物和ABG部分发生反应，TSG - PEI@ABG水凝胶迅速形成，其结构和性能可定制。基于TSG - PEI网络与ABG部分在纳米尺度上的紧密整合，这种水凝胶对组织表现出强大的粘附力以及工程支架的持久稳定性。因此，其自身具备的生物相容性、高粘附强度、压缩模量和成骨能力使其在调节骨髓间充质干细胞（BMSC）增殖和分化方面具有显著能力，这可能为组织工程领域提供一种同时实现支架固定和促进骨再生的潜在策略。
