Huang Ssu-Meng, Chen Wen-Cheng, Wu Cheng-Chen, Liu Shih-Ming, Ko Chia-Ling, Chen Jian-Chih, Shih Chi-Jen
Advanced Medical Devices and Composites Laboratory, Department of Fiber and Composite Materials, Feng Chia University, Taichung 407, Taiwan.
Advanced Medical Devices and Composites Laboratory, Department of Fiber and Composite Materials, Feng Chia University, Taichung 407, Taiwan; Department of Fragrance and Cosmetic Science, College of Pharmacy, Kaohsiung Medical University, Kaohsiung 807, Taiwan; Dental Medical Devices and Materials Research Center, College of Dental Medicine, Kaohsiung Medical University, Kaohsiung 807, Taiwan.
Biomater Adv. 2023 Sep;152:213524. doi: 10.1016/j.bioadv.2023.213524. Epub 2023 Jun 14.
Calcium phosphate bone cements (CPC) can be used in minimally invasive surgery because of their injectability, and they can also be used to repair small and irregular bone defects. This study aimed to release the antibiotic gentamicin sulfate (Genta) to reduce tissue inflammation and prevent infection in the early stages of bone recovery. Subsequently, the sustained release of the bone-promoting drug ferulic acid (FA) mimicked the response of osteoprogenitor D1 cells interaction, thereby accelerating the healing process of the overall bone repair. Accordingly, the different particle properties of micro-nano hybrid mesoporous bioactive glass (MBG), namely, micro-sized MBG (mMBG) and nano-sized MBG (nMBG), were explored separately to generate different dose releases in MBG/CPC composite bone cement. Results show that nMBG had better sustained-release ability than mMBG when impregnated with the same dose. When 10 wt% of mMBG hybrid nMBG and composite CPC were used, the amount of MBG slightly shortened the working/setting time and lowered the strength but did not hinder the biocompatibility, injectability, anti-disintegration, and phase transformation of the composite bone cement. Furthermore, compared with 2.5wt%Genta@mMBG/7.5 wt% FA@nMBG/CPC, 5wt.%Genta@mMBG/5wt.%FA@nMBG/CPC exhibited better antibacterial activity, better compressive strength, stronger mineralization of osteoprogenitor cell, and similar 14-day slow-release trend of FA. The MBG/CPC composite bone cement developed can be used in clinical surgery to achieve the synergistic sustained release of antibacterial and osteoconductive activities.
磷酸钙骨水泥（CPC）因其可注射性可用于微创手术，还可用于修复小的不规则骨缺损。本研究旨在释放抗生素硫酸庆大霉素（庆大霉素）以减轻组织炎症并预防骨恢复早期的感染。随后，促骨药物阿魏酸（FA）的持续释放模拟了骨祖细胞D1细胞相互作用的反应，从而加速了整体骨修复的愈合过程。因此，分别探索了微纳米混合介孔生物活性玻璃（MBG）的不同颗粒特性，即微米级MBG（mMBG）和纳米级MBG（nMBG），以在MBG/CPC复合骨水泥中产生不同剂量的释放。结果表明，当以相同剂量浸渍时，nMBG比mMBG具有更好的缓释能力。当使用10 wt%的mMBG与nMBG混合以及复合CPC时，MBG的量略微缩短了工作/凝固时间并降低了强度，但并未妨碍复合骨水泥的生物相容性、可注射性、抗崩解性和相变。此外，与2.5wt%庆大霉素@mMBG/7.5 wt% FA@nMBG/CPC相比，5wt.%庆大霉素@mMBG/5wt.%FA@nMBG/CPC表现出更好的抗菌活性、更好的抗压强度、更强的骨祖细胞矿化能力以及相似的FA 14天缓释趋势。所开发的MBG/CPC复合骨水泥可用于临床手术，以实现抗菌和骨传导活性的协同持续释放。
