Wen Huang, Poutiainen Pekka, Batnasan Enkhzaya, Latonen Leena, Lehto Vesa-Pekka, Xu Wujun
Department of Technical Physics, University of Eastern Finland, Yliopistonranta 1F, 70211 Kuopio, Finland.
Kuopio University Hospital, University of Eastern Finland, Puijonlaaksontie 2, 70210 Kuopio, Finland.
Pharmaceutics. 2023 Oct 22;15(10):2507. doi: 10.3390/pharmaceutics15102507.
Mesoporous silicon nanoparticles (PSi NPs) are promising platforms of nanomedicine because of their good compatibility, high payload capacities of anticancer drugs, and easy chemical modification. Here, PSi surfaces were functionalized with bisphosphonates (BP) for radiolabeling, loaded with doxorubicin (DOX) for chemotherapy, and the NPs were coated with cancer cell membrane (CCm) for homotypic cancer targeting. To enhance the CCm coating, the NP surfaces were covered with polyethylene glycol prior to the CCm coating. The effects of the BP amount and pH conditions on the radiolabeling efficacy were studied. The maximum BP was (2.27 wt%) on the PSi surfaces, and higher radiochemical yields were obtained for Tc (97% ± 2%) and Ga (94.6% ± 0.2%) under optimized pH conditions (pH = 5). The biomimetic NPs exhibited a good radiochemical and colloidal stability in phosphate-buffered saline and cell medium. In vitro studies demonstrated that the biomimetic NPs exhibited an enhanced cellular uptake and increased delivery of DOX to cancer cells, resulting in better chemotherapy than free DOX or pure NPs. Altogether, these findings indicate the potential of the developed platform for cancer treatment and diagnosis.
介孔硅纳米颗粒（PSi NPs）因其良好的兼容性、抗癌药物的高负载能力以及易于化学修饰，而成为纳米医学中很有前景的平台。在此，用双膦酸盐（BP）对PSi表面进行功能化以用于放射性标记，负载阿霉素（DOX）用于化疗，并用癌细胞膜（CCm）包覆纳米颗粒用于同型癌症靶向。为增强CCm包覆效果，在CCm包覆之前先用聚乙二醇覆盖纳米颗粒表面。研究了BP用量和pH条件对放射性标记效率的影响。PSi表面的最大BP含量为（2.27 wt%），在优化的pH条件（pH = 5）下，Tc（97% ± 2%）和Ga（94.6% ± 0.2%）获得了更高的放射化学产率。仿生纳米颗粒在磷酸盐缓冲盐水和细胞培养基中表现出良好的放射化学稳定性和胶体稳定性。体外研究表明，仿生纳米颗粒表现出增强的细胞摄取能力以及向癌细胞递送DOX的量增加，从而产生比游离DOX或纯纳米颗粒更好的化疗效果。总之，这些发现表明所开发的平台在癌症治疗和诊断方面具有潜力。
