Promdontree Praewa, Kheolamai Pakpoom, Ounkaew Artjima, Narain Ravin, Ummartyotin Sarute
Department of Materials and Textile Technology, Faculty of Science and Technology, Thammasat University, Pathumthani 12121, Thailand.
Division of Cell Biology, Department of Preclinical Sciences, Faculty of Medicine, Thammasat University, Pathumthani 12120, Thailand.
Polymers (Basel). 2023 Oct 16;15(20):4098. doi: 10.3390/polym15204098.
Cellulose nanocrystals (CNCs) were successfully extracted and purified from hemp using an alkaline treatment and bleaching process and subsequently used in conjunction with polyvinyl alcohol to form a composite hydrogel. Cellulose nanocrystals (1-10% (/)) were integrated into polyvinyl alcohol, and sodium tetraborate (borax) was employed as a crosslinking agent. Due to the small number of cellulose nanocrystals, no significant peak change was observed in the FT-IR spectra compared to pristine polyvinyl alcohol. The porosity was created upon the removal of the water molecules, and the material was thermally stable up to 200 °C. With the presence of cellulose nanocrystals, the melting temperature was slightly shifted to a higher temperature, while the glass transition temperature remained practically unchanged. The swelling behavior was examined for 180 min in deionized water and PBS solution (pH 7.4) at 37 °C. The degree of swelling of the composite with cellulose nanocrystals was found to be higher than that of pristine PVA hydrogel. The cell viability (%) of the prepared hydrogel with different proportions of cellulose nanocrystals was higher than that of pristine PVA hydrogel. Based on the results, the prepared composite hydrogels from cellulose nanocrystals extracted from hemp and polyvinyl alcohol were revealed to be an excellent candidate for scaffold material for medical usage.
通过碱处理和漂白工艺成功地从大麻中提取并纯化了纤维素纳米晶体（CNCs），随后将其与聚乙烯醇结合使用以形成复合水凝胶。将纤维素纳米晶体（1 - 10%（/））整合到聚乙烯醇中，并使用硼酸钠（硼砂）作为交联剂。由于纤维素纳米晶体的数量较少，与原始聚乙烯醇相比，在傅里叶变换红外光谱（FT - IR）中未观察到明显的峰变化。在去除水分子后形成了孔隙，并且该材料在高达200°C的温度下具有热稳定性。在纤维素纳米晶体存在的情况下，熔点温度略有升高，而玻璃化转变温度基本保持不变。在37°C下于去离子水和PBS溶液（pH 7.4）中对溶胀行为进行了180分钟的检测。发现含有纤维素纳米晶体的复合材料的溶胀度高于原始PVA水凝胶。不同比例纤维素纳米晶体的制备水凝胶的细胞活力（%）高于原始PVA水凝胶。基于这些结果，由从大麻中提取的纤维素纳米晶体和聚乙烯醇制备的复合水凝胶被证明是用于医疗用途的支架材料的极佳候选者。
