Department of Chemistry and Chemical Processing Technologies, Vocational School of Technical Sciences, Gazi University, Ankara, Turkey.
Department of Material and Material Processing Technologies, Vocational School of Technical Sciences, Gazi University, Ankara, Turkey.
Biotechnol J. 2024 Jan;19(1):e2300147. doi: 10.1002/biot.202300147. Epub 2023 Nov 7.
BACKGROUND/AIMS: Natural and synthetic biocompatible polymers have received significant attention in the pharmaceutical industry due to their rapid and effective healing properties in the wound healing process. The aim of this study was to optimize the extraction of onions, the preparation of sodium alginate/collagen/hydrogen boron nitride (NaAlg/Col/h-BN) membranes using the Box-Behnken experimental design, and determine the optimal conditions for quercetin release. The study also aimed to investigate the antimicrobial and antioxidant activities of the prepared membranes and their therapeutic properties.
The prepared membranes were characterized by scanning electron microscopy (SEM), fourier transform infrared (FTIR), differential scanning calorimetry (DSC), and X-ray diffraction (XRD). Antimicrobial activities were tested against Gram-negative (Gr-) Escherichia coli ATCC 25922, Klebsiella pneumonia, Enterobacter aerogenes, Gram-positive (Gr+) Staphylococcus aureus ATCC 25923, and Candida albicans ATCC 10231 pathogens. In vitro release studies were conducted to examine the therapeutic properties of the prepared membranes. The optimum conditions for the extraction of onions and the preparation of NaAlg/Col/h-BN membranes were found to be EtOH = 75 mL, t = 2 h, T = 45°C, and NaAlg = 1.0 g, Col = 2.0 g, and h-BN = 6% wt, respectively. The prepared membranes exhibited serious antimicrobial properties against S. aureus and C. albicans. The membranes also promoted the controlled release of quercetin for 24 h in vitro, indicating their potential as a new approach in wound treatment.
The study concludes that quercetin-filled NaAlg/Col/h-BN membranes have promising therapeutic properties for wound healing. The membranes exhibited significant antimicrobial and antioxidant properties, and their controlled release of quercetin suggests their potential for use in wound healing applications.
背景/目的：由于天然和合成生物相容性聚合物在伤口愈合过程中具有快速有效的愈合特性，因此在制药行业受到了广泛关注。本研究旨在优化洋葱的提取方法，使用 Box-Behnken 实验设计制备海藻酸钠/胶原/氮化硼（NaAlg/Col/h-BN）膜，并确定槲皮素释放的最佳条件。该研究还旨在研究制备的膜的抗菌和抗氧化活性及其治疗特性。
通过扫描电子显微镜（SEM）、傅里叶变换红外（FTIR）、差示扫描量热法（DSC）和 X 射线衍射（XRD）对制备的膜进行了表征。抗菌活性测试针对革兰氏阴性（Gr-）大肠杆菌 ATCC 25922、肺炎克雷伯菌、产气肠杆菌、革兰氏阳性（Gr+）金黄色葡萄球菌 ATCC 25923 和白色念珠菌 ATCC 10231 病原体。进行了体外释放研究，以检查制备的膜的治疗特性。发现洋葱的最佳提取条件和 NaAlg/Col/h-BN 膜的最佳制备条件分别为 EtOH = 75 mL、t = 2 h、T = 45°C 和 NaAlg = 1.0 g、Col = 2.0 g 和 h-BN = 6% wt。制备的膜对金黄色葡萄球菌和白色念珠菌表现出严重的抗菌性能。膜还促进了槲皮素在体外 24 小时的控释，表明它们在伤口治疗方面具有应用潜力。
本研究得出结论，槲皮素填充的 NaAlg/Col/h-BN 膜具有治疗伤口愈合的良好治疗特性。膜表现出显著的抗菌和抗氧化性能，其槲皮素的控释表明它们在伤口愈合应用中的潜在用途。
