Shi Yi-Ping, Song Yan-Tao, Peng Qing-Tian, Lyu Lin-You, Shen Yue, Qian Na, Wu Yun-Na
College of Environment and Resources, Dalian Minzu University, Dalian 116600, Liaoning, China.
College of Bioscience and Biotechnology, Shenyang Agricultural University, Shenyang 110866, China.
Ying Yong Sheng Tai Xue Bao. 2023 Oct;34(10):2655-2662. doi: 10.13287/j.1001-9332.202310.009.
To explore the effect of monoculture and mixture sowing artificial grassland on the photosynthetic characteristics of and , we determined the diurnal variation of photosynthetic properties of and under different treatments. The results showed that the diurnal changes of net photosynthetic rate, blade temperature and transpiration rate of and showed 'unimodal type' in monoculture, the stomatal conductance of showed 'unimodal type', and the stomatal conductance and water use efficiency of showed 'bimodal type'. Under the mixed sowing treatment, the diurnal changes of net photosynthetic rate, blade temperature and transpiration rate of and showed 'unimodal type', the stomatal conductance and water use efficiency of showed 'unimodal type', and the stomatal conductance of showed 'bimodal type'. The peak photosynthetic rate of under mixture was signi-ficantly higher than that under monoculture, being 17.72 and 13.65 μmol CO·m·s, respectively. Under monoculture and mixture sowing treatments, the chlorophyll content of was higher than that of , nitrogen content of the leaves of was lower than that of , and the nitrogen content in the leaves of mixture sowing was significantly higher than that of monoculture sowing , which were 27.60 and 22.55 g·kg, respectively. Net photosynthetic rates of and were significantly positively correlated with stomatal conductance and transpiration rate, and significantly negatively correlated with intercellular CO concentration under different planting methods. Net photosynthetic rate of was significantly positively correlated with blade temperature and water use efficiency. In summary, mixed sowing was beneficial to enhance nitrogen content of . Our results provided a theoretical basis for the response of the photosynthetic characteristics of forage to planting mode of artificial grassland.
为探究单播和混播人工草地对[草种1]和[草种2]光合特性的影响，我们测定了不同处理下[草种1]和[草种2]光合特性的日变化。结果表明，单播时[草种1]和[草种2]的净光合速率、叶片温度和蒸腾速率的日变化呈“单峰型”，[草种1]的气孔导度呈“单峰型”，[草种2]的气孔导度和水分利用效率呈“双峰型”。混播处理下，[草种1]和[草种2]的净光合速率、叶片温度和蒸腾速率的日变化呈“单峰型”，[草种1]的气孔导度和水分利用效率呈“单峰型”，[草种2]的气孔导度呈“双峰型”。混播时[草种1]的光合速率峰值显著高于单播，分别为17.72和13.65 μmol CO₂·m⁻²·s⁻¹。单播和混播处理下，[草种1]的叶绿素含量高于[草种2]，[草种2]叶片的氮含量低于[草种1]，混播[草种2]叶片的氮含量显著高于单播[草种2]，分别为27.60和22.55 g·kg⁻¹。不同种植方式下，[草种1]和[草种2]的净光合速率与气孔导度和蒸腾速率显著正相关，与胞间CO₂浓度显著负相关。[草种1]的净光合速率与叶片温度和水分利用效率显著正相关。综上所述，混播有利于提高[草种2]的氮含量。我们的研究结果为人工草地种植模式下牧草光合特性的响应提供了理论依据。
