Blauer G
Arch Biochem Biophys. 1986 Nov 15;251(1):306-14. doi: 10.1016/0003-9861(86)90078-0.
Circular dichroism (CD) and light-absorption spectra of the system ferriprotoporphyrin IX-(-)-quinine as measured at 26 to 27 degrees C in dilute aqueous solutions of both pH 7.4 and 11.5 are reported. The CD spectra changed significantly with time during measurements extending for many days. By CD titrations, a predominant mole ratio of 1:1 in the complex is indicated. Sedimentation velocity and viscosity measurements, carried out under similar conditions, suggest the formation of large and specific aggregates at both pH 7.4 and 11.5-12. The large CD bands observed in the Soret region indicate chiral interactions between FP molecules arrayed within aggregates of FP-Q complexes. The observed time dependence of the ellipticities is considered to be due to dynamic changes in the steric arrangement of interacting units within the aggregates.
报道了在26至27摄氏度下，于pH 7.4和11.5的稀水溶液中测得的高铁原卟啉IX - (-)-奎宁体系的圆二色性(CD)和光吸收光谱。在长达数天的测量过程中，CD光谱随时间发生了显著变化。通过CD滴定表明，该络合物中主要的摩尔比为1:1。在相似条件下进行的沉降速度和粘度测量表明，在pH 7.4以及11.5 - 12时均形成了大的特定聚集体。在Soret区域观察到的大CD带表明，排列在FP - Q络合物聚集体内的FP分子之间存在手性相互作用。观察到的椭圆率的时间依赖性被认为是由于聚集体内相互作用单元的空间排列的动态变化所致。
