Institute of Loess Plateau, Shanxi University, Taiyuan, 030006, China.
Environ Sci Pollut Res Int. 2023 Nov;30(54):115896-115906. doi: 10.1007/s11356-023-30653-y. Epub 2023 Oct 28.
The extensive application of carbon nanomaterials (CNMs) has attracted increasing studies concerned about its environmental impact. These studies focus on single exposure to CNMs, but repeated exposures with relatively low concentration are more likely to occur under actual exposure scenario. In this study, we studied the metabolic functional and structure of soil microorganism community under single and repeated exposures to multiwalled carbon nanotubes (MW), graphene (GR), and fullerene (C) by Biolog EcoPlates and high-throughput sequencing. Our findings revealed that repeated exposures to CNMs significantly increase the metabolic activity and diversity of the soil microbial community as compared with single exposure. Principal component and similarity analysis not only indicated that GR exerted a stronger effect on soil microbial diversity among three exposures compared to C and MW, but also revealed that the metabolic activity of the soil microbial community was more affected by the exposure scenarios of CNMs than the type of CNMs. These findings elucidated the effect of CNMs under different exposure scenarios on soil microorganism community, providing a new perspective on the risk assessment of nanomaterials.
碳纳米材料（CNMs）的广泛应用引起了越来越多的关于其环境影响的研究。这些研究集中在单一暴露于 CNMs 上，但在实际暴露情况下，更可能发生相对低浓度的重复暴露。在这项研究中，我们通过 Biolog EcoPlates 和高通量测序研究了单暴露和重复暴露于多壁碳纳米管（MW）、石墨烯（GR）和富勒烯（C）对土壤微生物群落的代谢功能和结构的影响。我们的研究结果表明，与单一暴露相比，重复暴露于 CNMs 显著增加了土壤微生物群落的代谢活性和多样性。主成分和相似性分析不仅表明，与 MW 和 C 相比，GR 在三种暴露中对土壤微生物多样性的影响更强，而且还表明，CNMs 的暴露情况对土壤微生物群落的代谢活性的影响大于 CNMs 的类型。这些发现阐明了不同暴露情况下 CNMs 对土壤微生物群落的影响，为纳米材料的风险评估提供了新的视角。
