University of Veterinary Medicine, Department for Obstetrics and Farm Animal Clinic, H-1078, István u. 2., Budapest, Hungary.
RougeVet Veterinary Practice, H-2351, Fő út 45. Alsónémedi, Hungary.
Theriogenology. 2024 Jan 15;214:201-205. doi: 10.1016/j.theriogenology.2023.10.014. Epub 2023 Oct 20.
Primiparous and multiparous dairy cattle were evaluated for pregnancy using both transrectal ultrasonography and a point-of-care pregnancy test (Alertys OnFarm Test), which measures pregnancy-associated glycoproteins through lateral diffusion, between Days 28-34 of pregnancy results were compared. A total of 637 animals were included in this study. Pregnancy was confirmed via manual palpation between Days 57-64. Data on parity, calving, and time of artificial insemination (AI) were also collected and evaluated. Overall the accuracy of the lateral diffusion test was 93.1% with 98.9% sensitivity, 88.7 % specificity, 86.8 % positive predictive value, and 99.1% negative predictive value. In heifers, the Alertys OnFarm Test had 100% sensitivity and 81.6% specificity. In contrast, the test had a sensitivity and specificity of 98.5 and 89.5%, respectively for multiparous cows. The pregnancy loss between early diagnosis and confirmation increased with parity. Heifers suffered losses as low as 2.6%, whereas animals in the third parity had significantly more losses (17.9%). Season also affected losses with spring pregnancy losses being considerably higher than autumn losses. The veterinary workload was also evaluated. Using the combined method of pregnancy testing, animals were first tested with the lateral diffusion test; then, the test-negative animals were scanned again, and the number of scanned animals was reduced. The results ranged between 3274% on farms. We concluded that veterinary workload could be reduced by using the point-of-care test. However, farm-level differences may have affected the results of the present study.
本研究旨在评估经直肠超声检查和即时检测（Alertys OnFarm 检测）这两种检测方法在奶牛妊娠诊断中的效果，该即时检测通过侧向扩散测量妊娠相关糖蛋白。妊娠第 28-34 天的检测结果与产后第 57-64 天的触诊结果进行了对比。研究共纳入 637 头奶牛，产犊后第 57-64 天进行人工授精（AI）。记录奶牛胎次、产犊情况和 AI 时间，并对数据进行分析。总体而言，侧向扩散检测的准确率为 93.1%，灵敏度为 98.9%，特异性为 88.7%，阳性预测值为 86.8%，阴性预测值为 99.1%。在小母牛中，Alertys OnFarm 检测的灵敏度为 100%，特异性为 81.6%。相比之下，经产奶牛的灵敏度和特异性分别为 98.5%和 89.5%。从早期诊断到确认的妊娠损失随胎次增加而增加。小母牛的损失率低至 2.6%，而第三胎次的动物损失率显著更高（17.9%）。季节也会影响妊娠损失，春季的妊娠损失明显高于秋季。同时，我们还评估了兽医的工作量。使用联合妊娠检测方法，首先用侧向扩散检测对奶牛进行检测；然后，对检测为阴性的动物再次进行扫描，并减少扫描动物的数量。不同牧场的结果范围在 3274%之间。综上，即时检测可减少兽医工作量，但本研究中不同牧场之间可能存在差异。
