支链2-氧代酸脱氢酶会干扰肝脏丙酮酸脱氢酶活性及活性状态的测定。
Branched-chain 2-oxo acid dehydrogenase interferes with the measurement of the activity and activity state of hepatic pyruvate dehydrogenase.
作者信息
Goodwin G W, Paxton R, Gillim S E, Harris R A
出版信息
Biochem J. 1986 May 15;236(1):111-4. doi: 10.1042/bj2360111.
Abstract
Oxidative decarboxylation of pyruvate by branched-chain 2-oxo acid dehydrogenase can result in overestimation of the expressed and total activity of hepatic pyruvate dehydrogenase. Pyruvate is a poor substrate for branched-chain 2-oxo acid dehydrogenase relative to the branched-chain oxo acids; however, the comparable total activities of the two complexes in liver, the much greater activity state of branched-chain 2-oxo acid dehydrogenase compared with pyruvate dehydrogenase in most physiological states, and the use of high pyruvate concentrations, explain the interference that can occur in conventional radiochemical or indicator-enzyme linked assays of pyruvate dehydrogenase. Goat antibody that specifically inhibited branched-chain 2-oxo acid dehydrogenase was used in this study to provide a more specific assay for pyruvate dehydrogenase.
摘要
支链2-氧代酸脱氢酶对丙酮酸的氧化脱羧作用可能导致对肝丙酮酸脱氢酶表达活性和总活性的高估。相对于支链氧代酸而言，丙酮酸是支链2-氧代酸脱氢酶的不良底物；然而，肝脏中这两种复合物的总活性相当，在大多数生理状态下支链2-氧代酸脱氢酶的活性状态比丙酮酸脱氢酶高得多，以及使用高浓度丙酮酸，这些因素解释了在传统放射化学法或指示酶联法测定丙酮酸脱氢酶时可能出现的干扰。本研究使用特异性抑制支链2-氧代酸脱氢酶的山羊抗体来提供一种更特异的丙酮酸脱氢酶测定方法。
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