支链2-氧代酸脱氢酶复合物对4-甲硫基-2-氧代丁酸的氧化脱羧作用。
Oxidative decarboxylation of 4-methylthio-2-oxobutyrate by branched-chain 2-oxo acid dehydrogenase complex.
作者信息
Jones S M, Yeaman S J
出版信息
Biochem J. 1986 Jul 15;237(2):621-3. doi: 10.1042/bj2370621.
Abstract
Highly purified branched-chain 2-oxo acid dehydrogenase complex (BCOADC) oxidizes 4-methylthio-2-oxobutyrate and 2-oxobutyrate, with Km values of 67 microM and 18 microM respectively. The Vmax. for oxidation of these substrates is 27% and 53% respectively of that for 3-methyl-2-oxobutyrate. Highly purified pyruvate dehydrogenase complex (PDC) oxidizes 2-oxobutyrate (Km 100 microM; Vmax. 49% of that for pyruvate) but not 4-methylthio-2-oxobutyrate, whereas 2-oxoglutarate dehydrogenase complex will not utilize either 2-oxo acid as substrate. BCOADC kinase is inhibited by both 4-methylthio-2-oxobutyrate and 2-oxobutyrate, with half-maximal inhibition by 45 microM and 50 microM respectively. Phosphorylation of BCOADC in isolated adipocytes is inhibited by both 4-methylthio-2-oxobutyrate and 2-oxobutyrate, consistent with their inhibitory action of BCOADC kinase. Phosphorylation of PDC is decreased by 2-oxobutyrate, but not by 4-methylthio-2-oxobutyrate.
摘要
高度纯化的支链2-氧代酸脱氢酶复合物（BCOADC）可氧化4-甲硫基-2-氧代丁酸酯和2-氧代丁酸酯，其Km值分别为67微摩尔/升和18微摩尔/升。这些底物氧化反应的最大反应速度（Vmax）分别为3-甲基-2-氧代丁酸酯氧化反应最大反应速度的27%和53%。高度纯化的丙酮酸脱氢酶复合物（PDC）可氧化2-氧代丁酸酯（Km为100微摩尔/升；Vmax为丙酮酸氧化反应最大反应速度的49%），但不能氧化4-甲硫基-2-氧代丁酸酯，而2-氧代戊二酸脱氢酶复合物则不能将这两种2-氧代酸作为底物。BCOADC激酶受到4-甲硫基-2-氧代丁酸酯和2-氧代丁酸酯的抑制，半最大抑制浓度分别为45微摩尔/升和50微摩尔/升。在分离的脂肪细胞中，4-甲硫基-2-氧代丁酸酯和2-氧代丁酸酯均抑制BCOADC的磷酸化，这与其对BCOADC激酶的抑制作用一致。2-氧代丁酸酯可降低PDC的磷酸化，但4-甲硫基-2-氧代丁酸酯则无此作用。
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本文引用的文献
1
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Biochem J. 1980 Oct 1;191(1):147-54. doi: 10.1042/bj1910147.
2
Regulation of the branched chain 2-oxoacid dehydrogenase kinase reaction.支链2-氧代酸脱氢酶激酶反应的调节
FEBS Lett. 1982 Jul 19;144(1):57-62. doi: 10.1016/0014-5793(82)80568-1.
3
Rapid purification of bovine kidney branched-chain 2-oxoacid dehydrogenase complex containing endogenous kinase activity.快速纯化含有内源性激酶活性的牛肾支链2-氧代酸脱氢酶复合物。
FEBS Lett. 1983 Jun 27;157(1):54-8. doi: 10.1016/0014-5793(83)81115-6.
4
Regulation of branched-chain alpha-ketoacid dehydrogenase kinase.支链α-酮酸脱氢酶激酶的调节
Arch Biochem Biophys. 1984 May 15;231(1):48-57. doi: 10.1016/0003-9861(84)90361-8.
5
Purification and properties of pyruvate dehydrogenase kinase from bovine kidney.
J Biol Chem. 1983 Aug 10;258(15):9454-8.
6
Phosphorylation of branched-chain 2-oxo acid dehydrogenase complex in isolated adipocytes. Effects of 2-oxo acids.分离脂肪细胞中支链2-氧代酸脱氢酶复合物的磷酸化作用。2-氧代酸的影响。
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7
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8
cAMP-dependent protein kinase and lipolysis in rat adipocytes. I. Cell preparation, manipulation, and predictability in behavior.
J Biol Chem. 1985 Dec 5;260(28):15122-9.
9
Purification and characterization of branched chain alpha-keto acid dehydrogenase complex of bovine kidney.牛肾支链α-酮酸脱氢酶复合体的纯化与特性分析
Proc Natl Acad Sci U S A. 1978 Oct;75(10):4881-5. doi: 10.1073/pnas.75.10.4881.
10
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