绵羊肝脏5,10-亚甲基四氢叶酸还原酶酶活性必需氨基酸残基的鉴定
Identification of amino acid residues essential for enzyme activity of sheep liver 5,10-methylenetetrahydrofolate reductase.
作者信息
Varalakshmi K, Savithri H S, Rao N A
出版信息
Biochem J. 1986 May 15;236(1):295-8. doi: 10.1042/bj2360295.
Abstract
Sheep liver 5,10-methylenetetrahydrofolate reductase was subjected to specific chemical modification with phenylglyoxal, diethyl pyrocarbonate and N-bromosuccinimide. The second-order rate constants for inactivation were calculated to be 54 M-1 X min-1, 103 M-1 X min-1 and 154 M-1 X min-1 respectively. This inactivation could be prevented by incubation with substrates or products, suggesting that the residues modified, namely arginine, histidine and tryptophan, are essential for enzyme activity.
摘要
用苯乙二醛、焦碳酸二乙酯和N-溴代琥珀酰亚胺对绵羊肝脏5,10-亚甲基四氢叶酸还原酶进行了特异性化学修饰。计算出的失活二级速率常数分别为54 M⁻¹×min⁻¹、103 M⁻¹×min⁻¹和154 M⁻¹×min⁻¹。与底物或产物一起温育可防止这种失活，这表明被修饰的残基，即精氨酸、组氨酸和色氨酸，对酶活性至关重要。
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本文引用的文献
1
Protein measurement with the Folin phenol reagent.使用福林酚试剂进行蛋白质测定。
J Biol Chem. 1951 Nov;193(1):265-75.
2
LACTIC DEHYDROGENASE. IX. EFFECT OF PHOTO-OXIDATION UPON ACTIVITY AND COMPLEX FORMATION.乳酸脱氢酶。IX. 光氧化对活性及复合物形成的影响。
J Biol Chem. 1963 Oct;238:3249-55.
3
Studies on the methylene/methyl interconversion catalyzed by methylenetetrahydrofolate reductase from pig liver.猪肝亚甲基四氢叶酸还原酶催化的亚甲基/甲基相互转化的研究。
Biochemistry. 1982 Aug 17;21(17):4165-71. doi: 10.1021/bi00260a038.
4
Evidence for an essential arginine residue at the active site of Escherichia coli acetate kinase.大肠杆菌乙酸激酶活性位点存在必需精氨酸残基的证据。
Biochim Biophys Acta. 1981 Jul 24;660(1):142-7. doi: 10.1016/0005-2744(81)90119-4.
5
The mechanism of transamination. Function of the histidyl residue at the active site of supernatant aspartate transaminase.转氨基作用的机制。上清液天冬氨酸转氨酶活性位点处组氨酰残基的功能。
J Biol Chem. 1970 Feb 25;245(4):806-13.
6
Flavin-protein interaction in egg white flavoprotein.蛋清黄素蛋白中的黄素 - 蛋白质相互作用
J Biochem. 1973 Jun;73(6):1233-42. doi: 10.1093/oxfordjournals.jbchem.a130196.
7
Essential arginyl residues in Escherichia coli alkaline phosphatase.大肠杆菌碱性磷酸酶中的必需精氨酰残基。
Biochemistry. 1974 Jul 2;13(14):2865-71. doi: 10.1021/bi00711a014.
8
Kinetic studies of the acylation of pig muscle D-glyceraldehyde 3-phosphate dehydrogenase by 1,3-diphosphoglycerate and of proton uptake and release in the overall enzyme mechanism.1,3-二磷酸甘油酸对猪肌肉D-甘油醛-3-磷酸脱氢酶的酰化动力学研究以及整个酶机制中质子的摄取与释放
Biochem J. 1973 Dec;135(4):695-703. doi: 10.1042/bj1350695.
9
A functional arginine residue in rabbit-muscle aldolase.兔肌肉醛缩酶中的一个功能性精氨酸残基。
Eur J Biochem. 1976 Nov 15;70(2):517-22. doi: 10.1111/j.1432-1033.1976.tb11043.x.
10
Arginyl residues and anion binding sites in proteins.蛋白质中的精氨酰残基与阴离子结合位点
Mol Cell Biochem. 1979 Jul 31;26(2):71-92. doi: 10.1007/BF00232886.
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