绵羊肝脏丝氨酸羟甲基转移酶活性位点残基的鉴定
Identification of active-site residues of sheep liver serine hydroxymethyltransferase.
作者信息
Manohar R, Appaji Rao N
出版信息
Biochem J. 1984 Dec 15;224(3):703-7. doi: 10.1042/bj2240703.
Abstract
Chemical modification of amino acid residues with phenylglyoxal, N-ethylmaleimide and diethyl pyrocarbonate indicated that at least one residue each of arginine, cysteine and histidine were essential for the activity of sheep liver serine hydroxymethyltransferase. The second-order rate constants for inactivation were calculated to be 0.016 mM-1 X min-1 for phenylglyoxal, 0.52 mM-1 X min-1 for N-ethylmaleimide and 0.06 mM-1 X min-1 for diethyl pyrocarbonate. Different rates of modification of these residues in the presence and in the absence of substrates and the cofactor pyridoxal 5'-phosphate as well as the spectra of the modified protein suggested that these residues might occur at the active site of the enzyme.
摘要
用苯乙二醛、N-乙基马来酰亚胺和焦碳酸二乙酯对氨基酸残基进行化学修饰表明，精氨酸、半胱氨酸和组氨酸各自至少有一个残基对绵羊肝脏丝氨酸羟甲基转移酶的活性至关重要。计算得出苯乙二醛的二级失活速率常数为0.016 mM-1·min-1，N-乙基马来酰亚胺为0.52 mM-1·min-1，焦碳酸二乙酯为0.06 mM-1·min-1。在有和没有底物以及辅因子磷酸吡哆醛存在的情况下，这些残基的修饰速率不同，以及修饰后蛋白质的光谱表明这些残基可能位于酶的活性位点。
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本文引用的文献
1
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J Biol Chem. 1951 Nov;193(1):265-75.
2
3
Microspectrophotometric measurements on single crystals of mitochondrial serine hydroxymethyltransferase.线粒体丝氨酸羟甲基转移酶单晶的显微分光光度测量。
J Biol Chem. 1981 Apr 25;256(8):3776-80.
4
Serine hydroxymethyltransferase.丝氨酸羟甲基转移酶
Adv Enzymol Relat Areas Mol Biol. 1982;53:83-112. doi: 10.1002/9780470122983.ch3.
5
Evidence for an essential arginine residue at the active site of Escherichia coli acetate kinase.大肠杆菌乙酸激酶活性位点存在必需精氨酸残基的证据。
Biochim Biophys Acta. 1981 Jul 24;660(1):142-7. doi: 10.1016/0005-2744(81)90119-4.
6
Serine transhydroxymethylase. Affinity of the active site for substrates, substrate analogues, and anions.丝氨酸转羟甲基酶。活性位点对底物、底物类似物及阴离子的亲和力。
J Biol Chem. 1971 Jun 25;246(12):3961-6.
7
Structure of methylguanidinium dihydrogenorthophosphate. A model compound for arginine-phosphate hydrogen bonding.磷酸二氢甲胍的结构。精氨酸 - 磷酸盐氢键的模型化合物。
J Am Chem Soc. 1973 Jul 25;95(15):4834-40. doi: 10.1021/ja00796a012.
8
Serine transhydroxymethylase. Subunit structure and the involvement of sulfhydryl groups in the activity of the enzyme.丝氨酸转羟甲基酶。亚基结构及巯基在酶活性中的作用。
J Biol Chem. 1973 Sep 25;248(18):6456-61.
9
Arginine as a substrate binding site in aspartate aminotransferase.精氨酸作为天冬氨酸转氨酶中的底物结合位点。
Biochem Biophys Res Commun. 1975 Nov 3;67(1):198-202. doi: 10.1016/0006-291x(75)90302-2.
10
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