Department of Otolaryngology, West China-Guang'an Hospital, Sichuan University, Guang'an, Sichuan, China.
Department of Otolaryngology, The People's Hospital of Dujiangyan, Dujiangyan, Sichuan, China.
PeerJ. 2023 Oct 24;11:e16263. doi: 10.7717/peerj.16263. eCollection 2023.
NTMT1, a transfer methylase that adds methyl groups to the N-terminus of proteins, has been identified as a critical player in tumor development and progression. However, its precise function in pan-cancer is still unclear. To gain a more comprehensive understanding of its role in cancer, we performed a thorough bioinformatics analysis.
To conduct our analysis, we gathered data from multiple sources, including RNA sequencing and clinical data from the TCGA database, protein expression data from the UALCAN and HPA databases, and single-cell expression data from the CancerSEA database. Additionally, we utilized TISIDB to investigate the interaction between the tumor and the immune system. To assess the impact of NTMT1 on the proliferation of SNU1076 cells, we performed a CCK8 assay. We also employed cellular immunofluorescence to detect DNA damage and used flow cytometry to measure tumor cell apoptosis.
Our analysis revealed that NTMT1 was significantly overexpressed in various types of tumors and that high levels of NTMT1 were associated with poor survival outcomes. Functional enrichment analysis indicated that NTMT1 may contribute to tumor development and progression by regulating pathways involved in cell proliferation and immune response. In addition, we found that knockdown of NTMT1 expression led to reduced cell proliferation, increased DNA damage, and enhanced apoptosis in HNSCC cells.
High expression of NTMT1 in tumors is associated with poor prognosis. The underlying regulatory mechanism of NTMT1 in cancer is complex, and it may be involved in both the promotion of tumor development and the inhibition of the tumor immune microenvironment.
NTMT1 是一种转移甲基酶，可将甲基添加到蛋白质的 N 端，已被确定为肿瘤发生和发展的关键因素。然而，其在泛癌中的精确功能尚不清楚。为了更全面地了解其在癌症中的作用，我们进行了深入的生物信息学分析。
为了进行分析，我们从多个来源收集了数据，包括 TCGA 数据库中的 RNA 测序和临床数据、UALCAN 和 HPA 数据库中的蛋白质表达数据以及 CancerSEA 数据库中的单细胞表达数据。此外，我们利用 TISIDB 研究了肿瘤与免疫系统之间的相互作用。为了评估 NTMT1 对 SNU1076 细胞增殖的影响，我们进行了 CCK8 测定。我们还使用细胞免疫荧光法检测 DNA 损伤，并使用流式细胞术测量肿瘤细胞凋亡。
我们的分析表明，NTMT1 在各种类型的肿瘤中均显著过表达，高水平的 NTMT1与不良的生存结局相关。功能富集分析表明，NTMT1 可能通过调节与细胞增殖和免疫反应相关的途径，促进肿瘤的发生和发展。此外，我们发现下调 NTMT1 表达可导致 HNSCC 细胞增殖减少、DNA 损伤增加和凋亡增强。
肿瘤中高表达的 NTMT1 与预后不良相关。NTMT1 在癌症中的潜在调节机制复杂，它可能参与肿瘤的促进和肿瘤免疫微环境的抑制。
