Pak J Biol Sci. 2023 Jan;26(7):347-359. doi: 10.3923/pjbs.2023.347.359.
<b>Background and Objective:</b> The flavor enhancer Monosodium Glutamate (MSG) is mostly utilized in Asian and West African cuisines, especially in West African and Asian dishes. However, due to its availability, largely without labeling, in many food products, unintentional overuse of this food additive may occur. The objective of this study was to find out how selenium nanoparticles affected the toxicity of MSG in male albino rats' testicles. <b>Materials and Methods:</b> As 35 Wistar male rats partitioned into 5 groups: G1: Control rats, G2: Received Se-NPs at 0.4 mg kg<sup>1</sup> b.wt., orally, G3: Injected with MSG at a daily dose of 4 g kg<sup>1</sup> b.wt., intraperitoneally (IP), G4: Ingested a daily oral dose of Se-NPs for 7 successive days and on the 7th day, received the first dose of MSG IP 4 g kg<sup>1</sup> b.wt., then received both treatments till the end of the study and G5: Administered a daily oral dose of 4 g kg<sup>1</sup> MSG, followed by Se-NPs at a daily dose of 0.4 mg kg<sup>1</sup> b.wt., the experiment continued for 28 days. Serum testosterone hormone, Follicle Stimulating Hormone (FSH), Luteinizing Hormone (LH), the levels of serum lipid peroxidation (MDA), reduced Glutathione (GSH), Glutathione Peroxidase (GSH-Px), superoxide dismutase (SOD) and Lactate Dehydrogenase (LDH) were estimated and samples from testis were separated for histological analysis. <b>Results:</b> The MSG treatment induced a significant decline in the values of serum testosterone, FSH, LH, GSH, GSH-Px and SOD. It also increased the values of serum MDA and LDH and spermatic arrest. While, the administration of Se-NPs orally before MSG treatment resulted in a decline in the values of serum MDA and LDH, an elevation in the values of serum GSH, GSH-PX and SOD, testosterone, FSH, LH and reappearance of sperm. <b>Conclusion:</b> The use of Se-NPs as a protector exhibited more improvement in values of estimated hormones and oxidative stress markers than using it as a therapy.
鲜味剂谷氨酸单钠（MSG）主要用于亚洲和西非菜肴，尤其是在西非和亚洲菜肴中。然而，由于其在许多食品中的广泛存在，且大部分情况下没有标签，因此可能会无意中过量使用这种食品添加剂。本研究的目的是了解硒纳米颗粒如何影响雄性白化大鼠睾丸中 MSG 的毒性。
将 35 只 Wistar 雄性大鼠分为 5 组：G1：对照组大鼠；G2：口服给予 0.4mg/kg·bw 的硒纳米颗粒；G3：每天腹腔注射 4g/kg·bw 的 MSG；G4：连续 7 天每天口服给予硒纳米颗粒，第 7 天给予首剂量腹腔注射 4g/kg·bw 的 MSG，然后继续两种处理直至研究结束；G5：每天口服给予 4g/kg·bw 的 MSG，然后给予 0.4mg/kg·bw 的硒纳米颗粒，实验持续 28 天。测定血清睾酮激素、卵泡刺激素（FSH）、黄体生成素（LH）、血清脂质过氧化（MDA）、还原型谷胱甘肽（GSH）、谷胱甘肽过氧化物酶（GSH-Px）、超氧化物歧化酶（SOD）和乳酸脱氢酶（LDH）水平，并分离睾丸组织样本进行组织学分析。
MSG 处理导致血清睾酮、FSH、LH、GSH、GSH-Px 和 SOD 值显著下降。它还增加了血清 MDA 和 LDH 值，并导致精子停滞。然而，在 MSG 处理前口服给予硒纳米颗粒导致血清 MDA 和 LDH 值下降，血清 GSH、GSH-PX 和 SOD 值升高，睾酮、FSH、LH 和精子重新出现。
作为一种保护剂，硒纳米颗粒的使用在改善估计激素和氧化应激标志物方面比作为一种治疗方法表现出更好的效果。
