Biochemistry Department, Faculty of science, Zagazig University, Zagazig, Egypt.
National Institute of Laser Enhanced Sciences, Cairo University, Giza, Egypt.
Food Chem Toxicol. 2023 Sep;179:113979. doi: 10.1016/j.fct.2023.113979. Epub 2023 Aug 6.
Cisplatin (Cis) is a treatment for testicular germ-cell tumors (TGCTs). Unfortunately, it causes testicular toxicity due to releasing reactive oxygen species (ROS) causing damage to testicular cells and chromosomes. The current study aimed to investigate the ameliorative effect of selenium nanoparticles (SeNPs) against cisplatin testicular toxicity in male rats by assessment of body weight, testis weight, oxidative stress markers in testis homogenates as (malondialdehyde (MDA), Superoxide dismutase (SOD), Glutathione reduced (GSH), Glutathione peroxidase (GSH ∼ PX) and Catalase (CAT)), gene expression, testosterone concentration (T), sperm characteristics (count, motility and abnormality) and testicular histopathology. Methods: Thirty adult male rats divided equally into four groups; a single dose intraperitoneally injection of cisplatin (10 mg/kg) and selenium nanoparticles (2 mg/kg/day) were administrated alone or in combination. Cis group showed a decrease in body weight, testis weight, antioxidant activities (SOD, GSH, GSH ∼ PX and CAT), T concentration and steroidogenetic expression, the data recorded an increase in MDA levels and sperm abnormality, meanwhile histopathology of testis sections showed degenerative changes in the seminiferous tubules. The co-administration of selenium nanoparticles ameliorated the harmful effects of cisplatin. In conclusion; SeNPs through its antioxidant potential may be useful to prevent the testicular toxicity induced by cisplatin to the rat testis by reducing oxidative stress.
顺铂（Cis）是治疗睾丸生殖细胞肿瘤（TGCT）的一种方法。不幸的是，它会因释放活性氧（ROS）而导致睾丸毒性，从而对睾丸细胞和染色体造成损伤。本研究旨在通过评估体重、睾丸重量、睾丸匀浆中的氧化应激标志物（丙二醛（MDA）、超氧化物歧化酶（SOD）、还原型谷胱甘肽（GSH）、谷胱甘肽过氧化物酶（GSH-PX）和过氧化氢酶（CAT））、基因表达、睾酮浓度（T）、精子特征（计数、活力和异常）和睾丸组织病理学来研究硒纳米粒子（SeNPs）对顺铂睾丸毒性的改善作用。方法：将 30 只成年雄性大鼠平均分为四组；单独或联合腹腔注射顺铂（10mg/kg）和硒纳米粒子（2mg/kg/天）。 Cis 组体重、睾丸重量、抗氧化活性（SOD、GSH、GSH-PX 和 CAT）、T 浓度和类固醇生成表达下降，MDA 水平和精子异常增加，同时睾丸切片组织病理学显示生精小管变性。硒纳米粒子的联合给药减轻了顺铂的有害影响。总之；SeNPs 通过其抗氧化潜力可能有助于通过减少氧化应激来预防顺铂对大鼠睾丸的毒性。
