Department of Agricultural Entomology, Tamil Nadu Agricultural University, Coimbatore, Tamil Nadu, India.
Department of Medicinal and Aromatic Crops, Tamil Nadu Agricultural University, Coimbatore, Tamil Nadu, India.
Environ Sci Pollut Res Int. 2023 Nov;30(54):116039-116051. doi: 10.1007/s11356-023-30522-8. Epub 2023 Oct 31.
Liquid chromatography mass spectrometry (LC-MS)-based detection of flonicamid, imidacloprid and 6-chloronicotinic acid residues was validated and analysed in capsicum fruit, processed products and soil. The standard concentrations (0.0025 to 0.25 μg mL) of insecticides had a good linear curve (r>0.99). Limit of detection and limit of quantification values were 0.0025 and 0.01 mg kg, respectively. The accuracy (80.53 to 100.33 %) of capsicum matrices and soil (89.41 to 100.52 %) and precision (RSD <10%) were established. Dissipation of imidacloprid (20 and 40 g a.i. ha) and flonicamid (75 and 150 g a.i. ha) at single (X) and double dose (2X) was studied under open field and polyhouse conditions. Under open field conditions, the flonicamid and imidacloprid residues persisted with half-life of 1.98, 2.90 days (X) and 2.80, 3.14 (2X) days, respectively. While under polyhouse conditions, the flonicamid and imidacloprid residues persisted with a half-life of 2.84, 3.66 (X) and 3.24, 3.97 (2X) days, respectively. The metabolite, 6-CNA, was not detected in any samples under open field and polyhouse condition. Among decontamination treatments, cooking in boiling water for 10 minutes reduced 78 to 81.60 percent of imidacloprid and flonicamid residues in both doses. The estimated dietary risk assessment of imidacloprid and flonicamid residues (RQ <1) indicated that the risk is within the acceptable limit. In farmgate capsicum samples, residues of flonicamid (7 samples) and imidacloprid (11 samples) were detected. Market samples of capsicum products (powder, flakes and sauce) were not detected with residues of selected insecticides.
基于液相色谱-质谱（LC-MS）的氟虫酰胺、吡虫啉和 6-氯烟碱酸残留检测方法在辣椒果实、加工产品和土壤中得到了验证和分析。标准浓度（0.0025 至 0.25μg/mL）的杀虫剂具有良好的线性曲线（r＞0.99）。检测限和定量限分别为 0.0025 和 0.01mg/kg。辣椒基质和土壤（89.41%至 100.52%）的准确度（80.53%至 100.33%）和精密度（RSD＜10%）均得到建立。在露天和温室条件下，研究了单（X）和双剂量（2X）施用量（20 和 40g a.i./ha）的吡虫啉和氟虫酰胺（75 和 150g a.i./ha）的消解情况。在露天条件下，氟虫酰胺和吡虫啉的半衰期分别为 1.98 天和 2.90 天（X）和 2.80 天和 3.14 天（2X）。而在温室条件下，氟虫酰胺和吡虫啉的半衰期分别为 2.84 天和 3.66 天（X）和 3.24 天和 3.97 天（2X）。在露天和温室条件下，均未检测到代谢物 6-CNA。在各种去污处理中，沸水中煮 10 分钟可减少 78%至 81.60%的单剂量和双剂量的吡虫啉和氟虫酰胺残留。吡虫啉和氟虫酰胺残留的估计膳食风险评估（RQ＜1）表明，风险处于可接受范围内。在农场门口的辣椒样品中，检测到 7 个样品的氟虫酰胺残留和 11 个样品的吡虫啉残留。辣椒粉、片状物和调味汁等辣椒产品的市场样品未检测到所选杀虫剂的残留。
