Meng Chen, Yan Bing, Huang Yuqing, Wang Chengshuo, Zhang Luo
Department of Otolaryngology，Head and Neck Surgery，Beijing TongRen Hospital，Capital Medical University，Beijing，100730，China.
Beijing Institute of Otolaryngology，Beijing Laboratory of Allergic Diseases，Beijing Key Laboratory of Nasal Diseases，Key Laboratory of Otolaryngology Head and Neck Surgery，Ministry of Education，Capital Medical University.
Lin Chuang Er Bi Yan Hou Tou Jing Wai Ke Za Zhi. 2023 Nov;37(11):897-901. doi: 10.13201/j.issn.2096-7993.2023.11.008.
To analyze the differential expression of neural precursor cell-expressed developmentally downregulated 8（NEDD8） protein in nasal polyp tissues of patients with different pathological types of chronic rhinorhinosinusitis with nasal polyps（CRSwNP）. All specimens were obtained from the specimen library of Beijing Tongren Hospital, and were all patients who underwent nasal endoscopic surgery for chronic rhinosinusitis in Beijing Tongren Hospital. Hematoxylin-eosin staining（HE） was used to detect the number of eosinophils in nasal polyps, and CRSwNP patients were grouped according to the number of eosinophils in nasal polyps, immunohistochemistry was used to detect and analyze the expression level of NEDD8 protein in nasal polyps. The expression level of NEDD8 protein in nasal polyps of patients with eosinophilic chronic rhinorhinosinusitis with nasal polyps was significantly higher than that of patients with non-eosinophilic chronic rhinosinusitis and nasal polyps（<0.05）. In addition, there was a significant positive correlation between the expression level of NEDD8 protein and the number of eosinophils in nasal polyp tissue（=0.79, =0.02）. There are differences in the expression of NEDD8 protein in patients with chronic rhinosinusitis and nasal polyps of different pathological types.
分析神经前体细胞表达的发育下调蛋白8（NEDD8）在不同病理类型慢性鼻-鼻窦炎伴鼻息肉（CRSwNP）患者鼻息肉组织中的差异表达。所有标本均取自北京同仁医院标本库，均为在北京同仁医院接受慢性鼻-鼻窦炎鼻内镜手术的患者。采用苏木精-伊红染色（HE）检测鼻息肉中嗜酸性粒细胞数量，根据鼻息肉中嗜酸性粒细胞数量对CRSwNP患者进行分组，采用免疫组织化学法检测并分析鼻息肉中NEDD8蛋白的表达水平。嗜酸性慢性鼻-鼻窦炎伴鼻息肉患者鼻息肉中NEDD8蛋白表达水平显著高于非嗜酸性慢性鼻-鼻窦炎伴鼻息肉患者（<0.05）。此外，NEDD8蛋白表达水平与鼻息肉组织中嗜酸性粒细胞数量呈显著正相关（=0.79，=0.02）。不同病理类型慢性鼻-鼻窦炎伴鼻息肉患者NEDD8蛋白表达存在差异。
