Key Laboratory of Animal Disease-Resistance Nutrition, Animal Nutrition Institute, Sichuan Agricultural University, Chengdu, China.
Key Laboratory of Animal Nutrition, College of Animal Science and Technology, Jiangxi Agricultural University, Nanchang, China.
Anim Biotechnol. 2023 Dec;34(8):3971-3977. doi: 10.1080/10495398.2023.2248200. Epub 2024 Jan 30.
This experiment was conducted to explore the effects of gut microbiota on neonatal diarrhea in a germ-free (GF) pig model. Twelve hysterectomy-derived GF piglets were housed in six sterile isolators. Among them, six piglets were treated as the GF group, and the other six piglets were orally introduced with healthy sow fecal suspension and regarded as the fecal microbiota transplantation (FMT) group. Another six piglets from natural birth were considered as the conventional (CV) group. The GF and FMT piglets were hand-fed with sterile milk powder for 21 days, and the CV piglets were suckled for the same days. Then, all piglets were fed with sterile feed for another 21 days. Results exhibited that the GF group's fecal score and moisture level were higher than those in the CV and FMT groups ( < 0.05). Meanwhile, the abundances of colonic AQP1 and AQP8 in the GF group were the greatest among these treatments ( < 0.05). However, FMT piglets had a lower fecal score in d 22-28 and d 29-35 than that in the CV piglets ( < 0.05). Collectively, the absence of gut microbiota may cause diarrhea in the piglet model, and transplantation of maternal fecal microbiota may reverse it.
本实验旨在通过无菌（GF）猪模型探索肠道微生物群对新生仔猪腹泻的影响。将 12 头剖腹产后的无菌仔猪饲养在 6 个无菌隔离器中。其中，6 头仔猪作为 GF 组，另外 6 头仔猪口服健康母猪粪便悬浮液，作为粪便微生物群移植（FMT）组。另外 6 头自然分娩的仔猪作为常规（CV）组。GF 和 FMT 仔猪用无菌奶粉人工喂养 21 天，CV 仔猪吮吸母乳相同天数。然后，所有仔猪再用无菌饲料喂养 21 天。结果表明，GF 组仔猪粪便评分和水分含量高于 CV 和 FMT 组（<0.05）。同时，GF 组结肠 AQP1 和 AQP8 的丰度在这些处理中最高（<0.05）。然而，FMT 仔猪在第 22-28 天和第 29-35 天的粪便评分低于 CV 仔猪（<0.05）。总之，肠道微生物群的缺失可能导致仔猪模型腹泻，而母体粪便微生物群的移植可能会逆转这种情况。
