Lee M T, Bruot B C, Adams W C
Biol Reprod. 1986 Oct;35(3):542-8. doi: 10.1095/biolreprod35.3.542.
The daily administration of human chorionic gonadotropin (hCG) to rats with thiouracil-induced hypothyroidism results in the development of cystic ovaries. This study was undertaken to delineate hormonal changes during the first 48 h of hCG treatment. Groups of euthyroid and hypothyroid rats were injected daily with hCG or saline for up to two days and killed at 0, 12, 24, or 48 h after the initial hCG injection. Sera were analyzed for progesterone (P), testosterone (T), 17 beta-estradiol (E2), and prolactin (Prl) by specific radioimmunoassay (RIA). Serum levels of these hormones were not significantly different in the euthyroid and hypothyroid rats. However, P was significantly elevated at 12, 24, and 48 h in the hypothyroid/hCG rats. T and Prl were significantly elevated at 12 and 48 h in the hypothyroid/hCG rats. T levels were also elevated at 12 and 48 h in the euthyroid rats receiving hCG. In contrast, hCG had no effect on P and Prl levels in the euthyroid rats. E2 levels were undetectable in the euthyroid and hypothyroid rats. The administration of hCG increased E2 in both the euthyroid and hypothyroid rats at 48 h with significantly more E2 detected in the hypothyroid rats. These results show that ovarian steroids and Prl levels increase during the early stages of cyst induction and suggest they may be important in triggering ovarian cyst formation.
对用硫脲嘧啶诱导甲状腺功能减退的大鼠每日给予人绒毛膜促性腺激素（hCG）会导致囊性卵巢的形成。本研究旨在描述hCG治疗最初48小时内的激素变化。将正常甲状腺和甲状腺功能减退的大鼠分组，每天注射hCG或生理盐水，持续两天，并在首次注射hCG后的0、12、24或48小时处死。通过特异性放射免疫测定法（RIA）分析血清中的孕酮（P）、睾酮（T）、17β-雌二醇（E2）和催乳素（Prl）。这些激素的血清水平在正常甲状腺和甲状腺功能减退的大鼠中无显著差异。然而，甲状腺功能减退/hCG组大鼠在12、24和48小时时P显著升高。甲状腺功能减退/hCG组大鼠在12和48小时时T和Prl显著升高。接受hCG的正常甲状腺大鼠在12和48小时时T水平也升高。相比之下，hCG对正常甲状腺大鼠的P和Prl水平无影响。正常甲状腺和甲状腺功能减退的大鼠中均未检测到E2水平。hCG给药后48小时，正常甲状腺和甲状腺功能减退的大鼠中E2均增加，且甲状腺功能减退的大鼠中检测到的E2显著更多。这些结果表明，在囊肿诱导的早期阶段卵巢类固醇和Prl水平升高，提示它们可能在触发卵巢囊肿形成中起重要作用。
