Department of Obstetrics and Gynecology, Center for Reproductive Medicine, Peking University Third Hospital, No. 49, Huayuan North Road, Haidian District, Beijing, 100191, People's Republic of China.
Endocrine. 2013 Feb;43(1):184-90. doi: 10.1007/s12020-012-9739-3. Epub 2012 Jul 14.
The objective of the present study was to characterize the effect of insulin plus hCG on the expression of steroidogenic enzymes (P450scc and CYP17) in polycystic ovaries of rats. Changes in estrous cycle, ovarian morphology, hormonal levels, and protein levels by immunohistochemistry and western-blot were determined. Rats treated with insulin plus hCG displayed abnormal estrous cycles with increasing androgen biosynthesis. Meanwhile, insulin plus hCG resulted in multiple large cysts with diminished granulosa layers and increased thecal layers and stromal-interstitial tissue. Moreover, there was an increase in the expression of P450scc and CYP17 in thecal and stromal cells in our PCOS rat model compared with control rats. These results indicate that administration of insulin with hCG can synergistically result in endogenous hyperandrogenism which may partially upregulate the expression of steroidogenic enzymes in ovarian tissue.
本研究旨在探讨胰岛素联合 hCG 对多囊卵巢大鼠类固醇生成酶（P450scc 和 CYP17）表达的影响。通过免疫组织化学和 Western-blot 检测动情周期、卵巢形态、激素水平和蛋白水平的变化。结果显示，胰岛素联合 hCG 处理的大鼠出现异常动情周期，同时伴随着雄激素生物合成的增加。此外，胰岛素联合 hCG 导致多个大卵泡形成，颗粒细胞层减少，膜细胞层和间质组织增加。与对照组相比，我们的 PCOS 大鼠模型中膜细胞和间质细胞中 P450scc 和 CYP17 的表达增加。这些结果表明，胰岛素联合 hCG 给药可协同导致内源性高雄激素血症，这可能部分上调卵巢组织中类固醇生成酶的表达。
