Slaper-Cortenbach I, Ploemacher R, Löwenberg B
Blood. 1987 Jan;69(1):135-9.
The factors determining the predominantly erythroid direction of human fetal liver hematopoiesis are unknown. We compared the capacities of human fetal liver and bone marrow stromas to sustain fetal and adult hematopoiesis in long-term cultures. In various marrow-fetal liver combinations of stroma and recharge, the maintenance of erythroid (BFU-e) and myeloid (CFU-GM) precursors in the nonadherent phase was determined. The morphology of the fetal liver nucleated cells during culture was also examined. This study shows that fetal liver stromas efficiently support fetal BFU-e for 6 to 7 weeks in vitro. Bone marrow stromas were not able to maintain fetal BFU-e beyond 4 weeks. Significant numbers of marrow BFU-e were not sustained in vitro on either source of stroma. On the other hand, the stroma layers of fetal liver and marrow origin were equally effective in maintaining fetal CFU-GM and adult CFU-GM in long-term culture. These findings show that the human embryonic liver stroma is a preferential site for stimulating fetal erythropoiesis. They do not demonstrate differences in stroma function to explain the relative paucity of myelopoiesis in the fetal liver.
决定人类胎儿肝脏造血主要向红细胞系方向发展的因素尚不清楚。我们比较了人类胎儿肝脏和骨髓基质在长期培养中维持胎儿及成人造血的能力。在基质和再接种的各种骨髓 - 胎儿肝脏组合中，测定了非贴壁期红细胞系（BFU - e）和髓系（CFU - GM）前体细胞的维持情况。还检查了培养过程中胎儿肝脏有核细胞的形态。本研究表明，胎儿肝脏基质在体外能有效支持胎儿BFU - e达6至7周。骨髓基质无法使胎儿BFU - e维持超过4周。无论哪种基质来源，大量的骨髓BFU - e在体外都无法维持。另一方面，胎儿肝脏和骨髓来源的基质层在长期培养中维持胎儿CFU - GM和成人CFU - GM的效果相同。这些发现表明，人类胚胎肝脏基质是刺激胎儿红细胞生成的优先部位。它们并未显示出基质功能上的差异来解释胎儿肝脏中髓系造血相对较少的原因。
