Jiao Yu-Ting, Kang Yi-Ran, Wen Ming-Yong, Wu Hui-Qian, Zhang Xin-Wei, Huang Wei-Hua
College of Chemistry and Molecular Sciences, Wuhan University, Wuhan, 430072, China.
Angew Chem Int Ed Engl. 2023 Dec 18;62(51):e202313612. doi: 10.1002/anie.202313612. Epub 2023 Nov 16.
The glutathione (GSH) system is one of the most powerful intracellular antioxidant systems for the elimination of reactive oxygen species (ROS) and maintaining cellular redox homeostasis. However, the rapid kinetics information (at the millisecond to the second level) during the dynamic antioxidation process of the GSH system remains unclear. As such, we specifically developed a novel dual-wire nanosensor (DWNS) that can selectively and synchronously measure the levels of GSH and ROS with high temporal resolution, and applied it to monitor the transient ROS generation as well as the rapid antioxidation process of the GSH system in individual cancer cells. These measurements revealed that the glutathione peroxidase (GPx) in the GSH system is rapidly initiated against ROS burst in a sub-second time scale, but the elimination process is short-lived, ending after a few seconds, while some ROS are still present in the cells. This study is expected to open new perspectives for understanding the GSH antioxidant system and studying some redox imbalance-related physiological.
谷胱甘肽（GSH）系统是用于清除活性氧（ROS）和维持细胞氧化还原稳态的最强大的细胞内抗氧化系统之一。然而，GSH系统动态抗氧化过程中的快速动力学信息（毫秒到秒级）仍不清楚。因此，我们专门开发了一种新型双丝纳米传感器（DWNS），它可以以高时间分辨率选择性地同步测量GSH和ROS的水平，并将其应用于监测单个癌细胞中瞬时ROS的产生以及GSH系统的快速抗氧化过程。这些测量结果表明，GSH系统中的谷胱甘肽过氧化物酶（GPx）在亚秒时间尺度上针对ROS爆发迅速启动，但清除过程是短暂的，几秒钟后就结束了，而细胞中仍存在一些ROS。这项研究有望为理解GSH抗氧化系统和研究一些与氧化还原失衡相关的生理学开辟新的视角。
