Department of Medical Research Center, State Key Laboratory for Complex Severe and Rare Diseases, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, China.
Department of Cardiology, State Key Laboratory for Complex Severe and Rare Diseases, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, China.
Exp Dermatol. 2023 Dec;32(12):2149-2159. doi: 10.1111/exd.14965. Epub 2023 Nov 1.
Bullous pemphigoid (BP) is a severe autoimmune blistering disease affecting patients' quality of life. Gut microbiota (GM) dysbiosis have been investigated to be associated with multiple autoimmune diseases. However, the relationship between GM and BP onset and remission remains to be established by a systematic study. We conducted a study that enrolled 24 patients with BP onset (BP group), 24 patients under remission stage (BP-R group) and 24 healthy controls (HC group). We applied 16S rRNA sequencing on faecal samples and revealed a separation of the microbiota structure. At the family level, Lachnospiraceae, Prevotellaceae and Veillonellaceae were more abundant in the HC and BP-R groups, while Bacteroidaceae, Ruminococcaceae and Enterobacteriaceae were more abundant in the BP group. Bugbase analysis revealed the potentially pathogenic bacteria had an increasing trend in the BP group compared with the HC group and this variation vanished in the BP-R group. At the amplicon sequence variants (ASV) level, Bacteroides ovatus (ASV40) and Veillonella dispar (ASV140) significantly decreased, while Prevotella copri (ASV54) increased in the BP group compared to the HC and BP-R groups. The HC group and BP-R group shared similar abundance. Furthermore, by correlation analysis, we investigated key ASVs correlated with clinical parameters and found some discriminate biomarkers between the BP and BP-R groups. Our study established a dynamic GM profile in BP patients under different disease activity, providing a new direction to understand the role of GM in BP pathogenesis and therapeutic effects.
大疱性类天疱疮（BP）是一种严重的自身免疫性水疱病，影响患者的生活质量。肠道微生物群（GM）失调与多种自身免疫性疾病有关。然而，GM 与 BP 发病和缓解之间的关系仍需要通过系统研究来确定。我们进行了一项研究，纳入了 24 名 BP 发病患者（BP 组）、24 名缓解期患者（BP-R 组）和 24 名健康对照者（HC 组）。我们对粪便样本进行了 16S rRNA 测序，揭示了微生物结构的分离。在科水平上，lachnospiraceae、Prevotellaceae 和 Veillonellaceae 在 HC 和 BP-R 组中更为丰富，而 Bacteroidaceae、Ruminococcaceae 和 Enterobacteriaceae 在 BP 组中更为丰富。Bugbase 分析显示，与 HC 组相比，BP 组中潜在的致病细菌呈增加趋势，而在 BP-R 组中这种变化消失了。在扩增子序列变异（ASV）水平上，与 HC 和 BP-R 组相比，Bacteroides ovatus（ASV40）和 Veillonella dispar（ASV140）显著减少，而 Prevotella copri（ASV54）增加。HC 组和 BP-R 组具有相似的丰度。此外，通过相关分析，我们研究了与临床参数相关的关键 ASV，并发现了 BP 和 BP-R 组之间的一些有区别的生物标志物。我们的研究建立了不同疾病活动状态下 BP 患者的动态 GM 图谱，为理解 GM 在 BP 发病机制和治疗效果中的作用提供了新的方向。
