Hu Haibei, Quan Guangqian, Yang Feng, Du Shan, Ding Siqin, Lun Yongzhi, Chen Qiang
Department of Thyroid and Breast Surgery, Shenzhen Hospital (Guangming), University of Chinese Academy of Sciences, Shenzhen, Guangdong, China.
Key Laboratory of Medical Microecology, Fujian Province University, School of Pharmacy and Medical Technology, Putian University, Putian, Fujian, China.
SAGE Open Med. 2023 Oct 29;11:20503121231205710. doi: 10.1177/20503121231205710. eCollection 2023.
Papillary thyroid cancer is the most common pathological type of thyroid cancer. miR-96-5p, a member of the miR-183 family, constitute a polycistronic miRNA cluster. In breast cancer, miR-96-5p promotes cell invasion, migration, and proliferation in vitro by inhibiting PTPN9. Moreover, miR-96-5p was reported to function as an oncogene in many cancers. However, whether miR-96-5p is involved in the development of papillary thyroid cancers and its potential mechanism is still unknown. The present study aims to explore the relationship between miR-96-5p and GPC3 expression in the development of papillary thyroid cancers.
Transcriptomic sequencing was carried out using six pairs of papillary thyroid cancer and adjacent normal tissues. Quantitative real-time polymerase chain reaction (PCR) experiments were performed to examine the expression of genes.
In total, there were 1588 up-regulated and 1803 down-regulated differentially expressed genes between papillary thyroid cancer and normal tissues. Gene ontology and Kyoto encyclopedia of genes and genomes analysis revealed that extracellular matrix structure and proteoglycans were mainly involved in papillary thyroid cancer. Among the cluster of proteoglycans, GPC3 was significantly down-regulated in papillary thyroid cancer and is a target of miR-96.
miR-96-5p participates in the development of papillary thyroid cancer by regulating the expression of GPC3. Thus, targeting miR-96-5p may be a potential therapeutic approach for preventing and treating papillary thyroid cancer.
甲状腺乳头状癌是甲状腺癌最常见的病理类型。miR-96-5p是miR-183家族的成员，构成一个多顺反子miRNA簇。在乳腺癌中，miR-96-5p通过抑制PTPN9促进体外细胞侵袭、迁移和增殖。此外，据报道miR-96-5p在许多癌症中发挥癌基因作用。然而，miR-96-5p是否参与甲状腺乳头状癌的发生及其潜在机制仍不清楚。本研究旨在探讨miR-96-5p与GPC3表达在甲状腺乳头状癌发生中的关系。
对6对甲状腺乳头状癌组织和癌旁正常组织进行转录组测序。采用定量实时聚合酶链反应（PCR）实验检测基因表达。
甲状腺乳头状癌组织与正常组织之间共有1588个上调和1803个下调的差异表达基因。基因本体论和京都基因与基因组百科全书分析显示，细胞外基质结构和蛋白聚糖主要参与甲状腺乳头状癌。在蛋白聚糖簇中，GPC3在甲状腺乳头状癌中显著下调，是miR-96的靶标。
miR-96-5p通过调节GPC3的表达参与甲状腺乳头状癌的发生。因此，靶向miR-96-5p可能是预防和治疗甲状腺乳头状癌的一种潜在治疗方法。
