Department of Urology, Shantou Central Hospital, Jinping District, Shantou 515031, China.
Curr Pharm Des. 2023;29(31):2477-2488. doi: 10.2174/0113816128254621231017062923.
microRNA-628-5p (miR-628-5p) has a significant impact on certain types of cancer. The precise function of miR-628-5p in the context of bladder urothelial carcinoma (BLCA) remains ambiguous.
We aimed to investigate the role of miR-628-5p in BLCA.
The samples were collected from The Cancer Genome Atlas (TCGA). Statistics were employed to evaluate the correlation and predictive significance of miR-628-5p. We analyzed the target genes and regulatory network of miR-628-5p and the correlation between miR-628-5p and immune infiltration. The expression of miR-628-5p in BLCA cells was confirmed by quantitative reverse-transcription PCR (qRT-PCR).
miR-628-5p exhibited differential expression across various types of cancer. There was a significant association between high expression of miR-628-5p and primary therapy outcome (p < 0.05). High expression of miR-628-5p was observed to be associated with poorer overall survival (HR: 1.42; 95% CI: 1.06-1.90; p = 0.02), progress free survival (HR: 1.57; 95% CI: 1.17-2.11; p = 0.003), and disease specific survival (HR: 1.83; 95% CI: 1.28-2.62; p = 0.001) in BLCA. miR-628-5p was an independent prognostic factor in BLCA and may be involved in the development of the disease through various pathways, including focal adhesion, ECM-receptor interaction, PI3K-Akt signaling pathway, and MAPK signaling pathway, and among others. miR-628-5p expression was significantly correlated with immune infiltration in BLCA patients. Compared to normal bladder epithelial cells, BLCA cell lines exhibited a significant upregulation of miR-628-5p.
It is possible that miR-628-5p could serve as a hopeful therapeutic target and prognostic biomarker for individuals with BLCA.
microRNA-628-5p（miR-628-5p）对某些类型的癌症有显著影响。miR-628-5p 在膀胱尿路上皮癌（BLCA）中的精确作用仍不清楚。
我们旨在研究 miR-628-5p 在 BLCA 中的作用。
本研究样本取自癌症基因组图谱（TCGA）。采用统计学方法评估 miR-628-5p 的相关性和预测意义。我们分析了 miR-628-5p 的靶基因和调控网络，以及 miR-628-5p 与免疫浸润的相关性。通过定量逆转录 PCR（qRT-PCR）验证 BLCA 细胞中 miR-628-5p 的表达。
miR-628-5p 在各种癌症中表现出差异表达。miR-628-5p 的高表达与原发治疗结局显著相关（p＜0.05）。miR-628-5p 高表达与总生存期（HR：1.42；95%CI：1.06-1.90；p=0.02）、无进展生存期（HR：1.57；95%CI：1.17-2.11；p=0.003）和疾病特异性生存期（HR：1.83；95%CI：1.28-2.62；p=0.001）较差相关。miR-628-5p 是 BLCA 的独立预后因素，可能通过多种途径参与疾病的发生发展，包括局灶黏附、ECM-受体相互作用、PI3K-Akt 信号通路和 MAPK 信号通路等。miR-628-5p 的表达与 BLCA 患者的免疫浸润显著相关。与正常膀胱上皮细胞相比，BLCA 细胞系中 miR-628-5p 的表达显著上调。
miR-628-5p 可能成为 BLCA 患者有希望的治疗靶点和预后生物标志物。
