Brownlie J
J Hyg (Lond). 1979 Aug;83(1):103-9. doi: 10.1017/s0022172400025869.
An infusion of E. coli endotoxin (10 microgram) into the mammary gland of the cow 16 h before experimental infection with St. agalactiae prevented the establishment of mastitis. A clinical examination of the affected gland did not reveal signs of inflammation, all organisms were eliminated from the milk by 36 h after infection. Mammary glands not pretreated with endotoxin, and injected with the same number of viable streptococci, showed signs of severe inflammation the milk yield was reduced and the injected pathogen could be isolated from the gland for up to 14 days when sampling was discontinued. The injection of endotoxin alone produced a rapid increase in the cell count of the milk, there were some signs of inflammation and 80% of the cells in the milk were neutrophils. The cell counts in the milk remained above normal for 7-10 days. Untreated control quarters appeared to be unaffected by the injection of E. coli endotoxin into an adjacent quarter, on the other hand the injection of St. agalactiae into the adjacent quarter on the same side of the udder produced a cell response which reached a peak after 2 days and returned to preinoculation levels by the fourth day. No inflammation was observed in these control quarters and no streptococci were isolated from the milk.
在感染无乳链球菌进行实验之前16小时，向奶牛乳腺中注入10微克大肠杆菌内毒素可预防乳腺炎的发生。对受影响乳腺的临床检查未发现炎症迹象，感染后36小时内所有微生物都从乳汁中清除。未用内毒素预处理且注射相同数量活链球菌的乳腺，出现了严重炎症迹象，产奶量降低，并且在停止采样前，在长达14天的时间里都能从乳腺中分离出注射的病原体。单独注射内毒素会使乳汁中的细胞计数迅速增加，有一些炎症迹象，乳汁中80%的细胞为中性粒细胞。乳汁中的细胞计数在7 - 10天内一直高于正常水平。未处理的对照乳腺似乎不受向相邻乳腺注射大肠杆菌内毒素的影响，另一方面，向同一侧乳房的相邻乳腺注射无乳链球菌会产生细胞反应，该反应在2天后达到峰值，并在第4天恢复到接种前水平。在这些对照乳腺中未观察到炎症，乳汁中也未分离出链球菌。
