A comprehensive review on genetic modification of plant cell wall for improved saccharification efficiency.

The focus is now on harnessing energy from green sources through sustainable technology to minimize environmental pollution. Several crop residues including rice and wheat straw are having enormous potential to be used as lignocellulosic source material for bioenergy production. The lignocellulosic feedstock is primarily composed of cellulose, hemicellulose, and lignin cell wall polymers. The hemicellulose and lignin polymers induce crosslinks in the cell wall, by firmly associating with cellulose microfibrils, and thereby, denying considerable access of cellulose to cellulase enzymes. This issue has been addressed by various researchers through downregulating several genes associated in monolignol biosynthesis in Arabidopsis, Poplar, Rice and Switchgrass to increase ethanol recovery. Similarly, xylan biosynthetic genes are also targeted to genetically culminate its accumulation in the secondary cell walls. Regulation of cellulose synthases (CesA) proves to be an effective tool in addressing the negative impact of these two factors. Modification in the expression of cellulose synthase aids in reducing cellulose crystallinity as well as polymerisation degree which in turn increases ethanol recovery. The engineered bioenergy crops and various fungal strains with state of art biomass processing techniques presents the most recent integrative biotechnology model for cost effective green fuels generation along with production of key value-added products with minuscule disturbances in the environment. Plant breeding strategies utilizing the existing variability for biomass traits will be key in developing dual purpose varieties. For this purpose, reorientation of conventional breeding techniques for incorporating useful biomass traits will be effective.