Nargessi R D, Landon J, Smith D S
J Immunol Methods. 1979;26(4):307-13. doi: 10.1016/0022-1759(79)90176-5.
Antibodies against the label are introduced as a potentially useful reagent in nonisotopic immunoassay. They may permit end point determination without the need for a separation step, provided (i) that steric hindrance selectively prevents their binding to the antibody-bound fraction of the labelled antigen in an immunoassay mixture, and (ii) that their binding to the label in the free fraction results in a change in its signal. This 'indirect' approach was investigated in systems employing the fluorescein label and antibodies to fluorescein which quenched the fluorescence of free labelled antigen. 'Indirect quenching' fluoroimmunoassays for human serum albumin, human immunoglobulin G and human placental lactogen were demonstrated. These assays for proteins may be contrasted with conventional non-separation techniques, which are usually best suited to the determination of haptens.
抗标记物抗体作为非同位素免疫分析中一种潜在有用的试剂被引入。如果（i）空间位阻能选择性地阻止它们与免疫分析混合物中抗体结合的标记抗原部分结合，以及（ii）它们与游离部分的标记物结合会导致其信号发生变化，那么它们可能允许在无需分离步骤的情况下进行终点测定。在使用荧光素标记物和抗荧光素抗体的系统中研究了这种“间接”方法，该抗体会淬灭游离标记抗原的荧光。已证实了用于人血清白蛋白、人免疫球蛋白G和人胎盘催乳素的“间接淬灭”荧光免疫分析。这些蛋白质分析方法可与传统的非分离技术形成对比，传统非分离技术通常最适合于半抗原的测定。
