Department of General Dentistry, Division of Interdisciplinary Dentistry, Faculty of Dental Science, Kyushu University, 3-1-1 Maidashi, Higashi-ku, Fukuoka, 812-8582, Japan.
Division of General Dentistry, Kyushu University Hospital, Kyushu University, 3-1-1 Maidashi, Higashi-ku, Fukuoka, 812-8582, Japan.
J Infect Chemother. 2024 Apr;30(4):306-314. doi: 10.1016/j.jiac.2023.10.021. Epub 2023 Nov 3.
Manuka oil and tea tree oil are essential oils with known antibacterial properties that are believed to be caused by one main component: terpinen-4-ol. Terpinen-4-ol has potent antibacterial activity against caries-related microorganisms. However, few studies have investigated the antimicrobial effects of terpinen-4-ol on bacteria in apical periodontitis. Thus, the objective of the present study was to evaluate the antibacterial and antibiofilm potential of terpinen-4-ol against Enterococcus faecalis, Porphyromonas gingivalis, Prevotella intermedia, and Fusobacterium nucleatum, which have all been detected in apical periodontitis. The minimum inhibitory and minimum bactericidal concentrations of terpinen-4-ol were determined to assess its activity against biofilms. The minimum inhibitory concentration of terpinen-4-ol was 0.25% against E. faecalis and F. nucleatum, 0.05% against P. gingivalis, and 0.1% against P. intermedia. The minimum bactericidal concentration of terpinen-4-ol was 1.0% against E. faecalis, 0.2% against P. gingivalis and P. intermedia, and 0.5% against F. nucleatum. In the biofilm evaluations, all terpinen-4-ol-treated bacteria had significant reductions in biofilm viability compared with controls in experiments assessing attachment inhibitory activity. Furthermore, structural alterations and decreased bacterial cell clumping were observed under scanning electron microscopy, and significantly decreased cell survival was noted using fluorescence microscopy. Together, these results suggest that terpinen-4-ol is a potential antibacterial agent with bactericidal properties, and can also act on established biofilms.
麦卢卡精油和茶树油是具有已知抗菌特性的精油，其被认为是由一种主要成分引起的：萜品-4-醇。萜品-4-醇对致龋相关微生物具有很强的抗菌活性。然而，很少有研究调查萜品-4-醇对根尖周炎中细菌的抗菌和抗生物膜作用。因此，本研究的目的是评估萜品-4-醇对已在根尖周炎中检测到的粪肠球菌、牙龈卟啉单胞菌、中间普氏菌和具核梭杆菌的抗菌和抗生物膜潜力。测定萜品-4-醇的最小抑菌和最小杀菌浓度，以评估其对生物膜的活性。萜品-4-醇对粪肠球菌和具核梭杆菌的最小抑菌浓度为 0.25%，对牙龈卟啉单胞菌为 0.05%，对中间普氏菌为 0.1%。萜品-4-醇的最小杀菌浓度对粪肠球菌为 1.0%，对牙龈卟啉单胞菌和中间普氏菌为 0.2%，对具核梭杆菌为 0.5%。在生物膜评估中，与对照相比，所有萜品-4-醇处理的细菌在评估附着抑制活性的实验中，生物膜活力均显著降低。此外，扫描电子显微镜下观察到结构改变和细菌细胞聚集减少，荧光显微镜下观察到细胞存活率显著降低。综上所述，这些结果表明萜品-4-醇是一种具有杀菌特性的潜在抗菌剂，也可以作用于已建立的生物膜。
