Maquera-Huacho Patricia Milagros, Tonon Caroline Coradi, Correia Marilia Ferreira, Francisconi Renata Serignoli, Bordini Ester Alves Ferreira, Marcantonio Élcio, Spolidorio Denise Madalena Palomari
a Department of Oral Diagnosis and Surgery, School of Dentistry , São Paulo State University (Unesp) , Araraquara , São Paulo , Brazil.
b Department of Physiology and Pathology, School of Dentistry , São Paulo State University (Unesp) , Araraquara, São Paulo , Brazil.
Biofouling. 2018 Jul;34(6):699-709. doi: 10.1080/08927014.2018.1485892. Epub 2018 Sep 6.
This study evaluated the antibacterial properties of carvacrol and terpinen-4-ol against Porphyromonas gingivalis and Fusobacterium nucleatum and its cytotoxic effects on fibroblast cells. The minimum inhibitory concentration (MIC) and the minimum bactericidal concentration (MBC) were examined. The minimum biofilm inhibition concentration (MBIC) was evaluated by XTT assay. Biofilm decontamination on titanium surfaces was quantified (CFU ml), evaluated by confocal laser scanning microscopy (CLSM) and cytotoxic activity by MTT. The MIC and MBC for carvacrol were 0.007% and 0.002% for P. gingivalis and F. nucleatum, and 0.06% for terpinen-4-ol for both microorganisms. The MBIC for carvacrol was 0.03% and 0.06% for P. gingivalis and F. nucleatum, and for terpinen-4-ol was 0.06% and 0.24%. The results indicated anti-biofilm activity using carvacrol (0.26%, 0.06%) and terpinen-4-ol (0.95%, 0.24%) and showed cytotoxic activity similar to chlorohexidine (CHX). However, terpinen-4-ol (0.24%) showed higher cell viability than other treatments. Carvacrol and terpinen-4-ol showed antibacterial activity in respect of reducing biofilms. Moreover, CHX-like cytotoxicity was observed.
本研究评估了香芹酚和萜品-4-醇对牙龈卟啉单胞菌和具核梭杆菌的抗菌特性及其对成纤维细胞的细胞毒性作用。检测了最低抑菌浓度（MIC）和最低杀菌浓度（MBC）。通过XTT法评估最低生物膜抑制浓度（MBIC）。对钛表面的生物膜去污进行定量（CFU/ml），通过共聚焦激光扫描显微镜（CLSM）进行评估，并通过MTT评估细胞毒性活性。香芹酚对牙龈卟啉单胞菌和具核梭杆菌的MIC和MBC分别为0.007%和0.002%，萜品-4-醇对这两种微生物的MIC和MBC均为0.06%。香芹酚对牙龈卟啉单胞菌和具核梭杆菌的MBIC分别为0.03%和0.06%，萜品-4-醇的MBIC分别为0.06%和0.24%。结果表明，香芹酚（0.26%，0.06%）和萜品-4-醇（0.95%，0.24%）具有抗生物膜活性，且显示出与氯己定（CHX）相似的细胞毒性活性。然而，萜品-4-醇（0.24%）显示出比其他处理更高的细胞活力。香芹酚和萜品-4-醇在减少生物膜方面表现出抗菌活性。此外，还观察到类似CHX的细胞毒性。
