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一种用于监测微钙化的2,7 - 二氯荧光素衍生物。

A 2,7-dichlorofluorescein derivative to monitor microcalcifications.

作者信息

Tholen Patrik, Brown Connor N, Keil Claudia, Bayir Ali, Zeng Hui-Hui, Haase Hajo, Thompson Richard B, Lengyel Imre, Yücesan Gündoğ

机构信息

Institute for Food Chemistry and Toxicology, Germany, Technische Universität Berlin, Gustav-Meyer-Allee 25, 13355 Berlin, Germany.

Wellcome-Wolfson Institute for Experimental Medicine, Queen's University Belfast, Belfast BT9 7BL, UK.

出版信息

Mol Syst Des Eng. 2022 Nov 1;7(11):1415-1421. doi: 10.1039/d2me00185c. Epub 2022 Oct 10.

DOI:10.1039/d2me00185c
PMID:37927331
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10624163/
Abstract

Herein, we report the crystal structure of 2,7-dichlorofluorescein methyl ester (DCF-ME) and its fluorescence response to hydroxyapatite binding. The reported fluorophore is very selective for staining the bone matrix and provides turn-on fluorescence upon hydroxyapatite binding. The reported fluorophore can readily pass the cell membrane of the C2C12 cell line, and it is non-toxic for the cell line. The reported fluorophore DCF-ME may find applications in monitoring bone remodeling and microcalcification as an early diagnosis tool for breast cancer and age-related macular degeneration.

摘要

在此，我们报道了2,7-二氯荧光素甲酯（DCF-ME）的晶体结构及其对羟基磷灰石结合的荧光响应。所报道的荧光团对骨基质染色具有高度选择性，并在与羟基磷灰石结合时产生开启荧光。所报道的荧光团能够轻易穿过C2C12细胞系的细胞膜，且对该细胞系无毒。所报道的荧光团DCF-ME作为乳腺癌和年龄相关性黄斑变性的早期诊断工具，可能在监测骨重塑和微钙化方面找到应用。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/947e/10624163/026f6901997a/nihms-1891701-f0005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/947e/10624163/81609ca9302f/nihms-1891701-f0001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/947e/10624163/d61318c1abaf/nihms-1891701-f0002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/947e/10624163/d1839954c661/nihms-1891701-f0003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/947e/10624163/3fe6c57a1900/nihms-1891701-f0004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/947e/10624163/026f6901997a/nihms-1891701-f0005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/947e/10624163/81609ca9302f/nihms-1891701-f0001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/947e/10624163/d61318c1abaf/nihms-1891701-f0002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/947e/10624163/d1839954c661/nihms-1891701-f0003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/947e/10624163/3fe6c57a1900/nihms-1891701-f0004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/947e/10624163/026f6901997a/nihms-1891701-f0005.jpg

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Fluorescent Arylphosphonic Acids: Synergic Interactions between Bone and the Fluorescent Core.荧光芳基膦酸：荧光核与骨之间的协同相互作用。
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The structures and gating mechanism of human calcium homeostasis modulator 2.
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Sci Rep. 2019 Nov 8;9(1):16327. doi: 10.1038/s41598-019-52808-z.
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Association of Microcalcification Clusters with Short-term Invasive Breast Cancer Risk and Breast Cancer Risk Factors.微钙化簇与短期浸润性乳腺癌风险及乳腺癌危险因素的相关性。
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A novel fluorescein-bisphosphonate based diagnostic tool for the detection of hydroxyapatite in both cell and tissue models.一种新型基于荧光素双磷酸酯的诊断工具，用于检测细胞和组织模型中的羟磷灰石。
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