一种用于监测微钙化的2,7 - 二氯荧光素衍生物。
A 2,7-dichlorofluorescein derivative to monitor microcalcifications.
作者信息
Tholen Patrik, Brown Connor N, Keil Claudia, Bayir Ali, Zeng Hui-Hui, Haase Hajo, Thompson Richard B, Lengyel Imre, Yücesan Gündoğ
机构信息
Institute for Food Chemistry and Toxicology, Germany, Technische Universität Berlin, Gustav-Meyer-Allee 25, 13355 Berlin, Germany.
Wellcome-Wolfson Institute for Experimental Medicine, Queen's University Belfast, Belfast BT9 7BL, UK.
出版信息
Mol Syst Des Eng. 2022 Nov 1;7(11):1415-1421. doi: 10.1039/d2me00185c. Epub 2022 Oct 10.
Herein, we report the crystal structure of 2,7-dichlorofluorescein methyl ester (DCF-ME) and its fluorescence response to hydroxyapatite binding. The reported fluorophore is very selective for staining the bone matrix and provides turn-on fluorescence upon hydroxyapatite binding. The reported fluorophore can readily pass the cell membrane of the C2C12 cell line, and it is non-toxic for the cell line. The reported fluorophore DCF-ME may find applications in monitoring bone remodeling and microcalcification as an early diagnosis tool for breast cancer and age-related macular degeneration.
在此，我们报道了2,7-二氯荧光素甲酯（DCF-ME）的晶体结构及其对羟基磷灰石结合的荧光响应。所报道的荧光团对骨基质染色具有高度选择性，并在与羟基磷灰石结合时产生开启荧光。所报道的荧光团能够轻易穿过C2C12细胞系的细胞膜，且对该细胞系无毒。所报道的荧光团DCF-ME作为乳腺癌和年龄相关性黄斑变性的早期诊断工具，可能在监测骨重塑和微钙化方面找到应用。
Zotero 插件