Research and Development Department, Japan BCG Laboratory, Kiyose, Tokyo, Japan.
Institute for Vaccine Research and Development, Hokkaido University, Sapporo, Hokkaido, Japan.
Front Immunol. 2023 Oct 18;14:1271228. doi: 10.3389/fimmu.2023.1271228. eCollection 2023.
bacilli Calmette-Guerin (BCG) is a licensed vaccine against tuberculosis. It requires attenuated live bacteria to be effective, possibly because actively secreted proteins play a critical role in inducing anti-tuberculosis immunity. BCG also functions as an effective adjuvant. Moreover, the effects of BCG components as adjuvants are not important as those of attenuated live BCG, which is used in cancer immunotherapy. However, the BCG secreted proteins have not been paid attention in anticancer immunity. To understand mycobacterial secreted proteins' function, we investigate immune responses to BCG culture filtrate proteins (CFP). Here, CFP strongly induce both antigen-specific CD4+ T cells and specific CD8+ T cells, which may be functional cytotoxic T lymphocytes (CTLs). In this study, we clearly demonstrate that CFP acts as an adjuvant for CTL induction against specific co-administered proteins and propose CFP as a new protein adjuvant. The CTL response shows potent anticancer effects in mice. These findings could provide insight into the contribution of mycobacterial secreted proteins in both anticancer and antimycobacterial immunity.
卡介苗（BCG）是一种用于预防结核病的已上市疫苗。它需要减毒活细菌才能发挥作用，这可能是因为其活跃分泌的蛋白在诱导抗结核病免疫方面发挥着关键作用。BCG 还具有有效的佐剂作用。此外，BCG 成分作为佐剂的效果不如用于癌症免疫治疗的减毒活 BCG 重要。然而，卡介苗分泌蛋白在抗癌免疫方面尚未得到重视。为了了解分枝杆菌分泌蛋白的功能，我们研究了针对卡介苗培养滤液蛋白（CFP）的免疫反应。在这里，CFP 强烈诱导抗原特异性 CD4+T 细胞和特异性 CD8+T 细胞，这些细胞可能是具有功能的细胞毒性 T 淋巴细胞（CTL）。在这项研究中，我们清楚地表明 CFP 作为一种佐剂，可增强针对特定共施用蛋白的 CTL 诱导，并提出 CFP 作为一种新的蛋白佐剂。CTL 反应在小鼠中显示出强大的抗癌作用。这些发现可以深入了解分枝杆菌分泌蛋白在抗癌和抗分枝杆菌免疫中的作用。
