Department of Chemistry, Case Western Reserve University, Cleveland, Ohio, USA.
Department of Mechanical and Aerospace Engineering, Case Western Reserve University, Cleveland, Ohio, USA.
Photochem Photobiol. 2024 May-Jun;100(3):530-540. doi: 10.1111/php.13878. Epub 2023 Nov 6.
The development of a suitable irradiation setup is essential for in vitro experiments in photodynamic therapy (PDT). While various irradiation systems have been developed for PDT, only a few offer practical and high-quality setups for precise and reproducible results in cell culture experiments. This report introduces a cost-effective illumination setup designed for in vitro photodynamic treatments. The setup consists of a commercially available light-emitting diode (LED) lamp, a cooling unit, and a specially designed 3D-printed enclosure to accommodate a multiwell plate insert. The LED lamp is versatile, supporting various irradiation wavelengths and adjustable illumination fields, ensuring consistent and reliable performance. The study evaluates the setup through various parameters, including photon flux density, illumination uniformity, photon distribution across the multiwell plate, and temperature changes during irradiation. In addition, the effectiveness of the LED-based illumination system is tested by treating mouse mammary breast carcinoma cells (4T1) with Rose Bengal and LED irradiation at around 525 nm. The resulting IC of 5.2 ± 0.9 μM and a minimum media temperature change of ca. 1.2°C indicate a highly promising LED-based setup that offers a cost-effective and technically feasible solution for achieving consistent, reproducible, and uniform irradiation, enhancing research capabilities and potential applications.
适合的辐照设置对于光动力疗法（PDT）的体外实验至关重要。虽然已经开发出了各种辐照系统，但只有少数系统为细胞培养实验提供了实用且高质量的设置，以实现精确和可重复的结果。本报告介绍了一种经济高效的用于体外光动力治疗的照明设置。该设置由市售的发光二极管（LED）灯、冷却单元和专门设计的 3D 打印外壳组成，可容纳多孔板插件。LED 灯用途广泛，支持各种辐照波长和可调的照明区域，确保了一致和可靠的性能。该研究通过各种参数评估了该设置，包括光子通量密度、照明均匀性、多孔板上的光子分布以及辐照过程中的温度变化。此外，还通过用孟加拉玫瑰红和大约 525nm 的 LED 辐照来测试基于 LED 的照明系统的有效性，以处理鼠乳腺乳腺癌细胞（4T1）。结果表明，IC 为 5.2±0.9μM，培养基温度变化最小约为 1.2°C，这表明基于 LED 的设置具有很大的潜力，提供了一种经济高效且技术可行的解决方案，可实现一致、可重复和均匀的辐照，增强了研究能力和潜在应用。
