SPAG5 通过激活 PI3K/AKT 信号通路促进结直肠癌细胞的增殖、迁移、侵袭和上皮-间充质转化。
SPAG5 promotes the proliferation, migration, invasion, and epithelial-mesenchymal transformation of colorectal cancer cells by activating the PI3K/AKT signaling pathway.
机构信息
Department of Gastroenterology, The First Affiliated Hospital of Chengdu Medical College, Chengdu, Sichuan, China.
Department of Traditional Chinese Medicine, Jinshan Hospital of Fudan University, Shanghai, China.
出版信息
Chin J Physiol. 2023 Sep-Oct;66(5):365-371. doi: 10.4103/cjop.CJOP-D-22-00165.
Colorectal cancer (CRC) is a cancer that occurs in the rectum or colon with a high incidence. Sperm-associated antigen 5 (SPAG5), a gene that regulates cell division, has been observed highly expressed in a variety of cancers, but its role in CRC is unclear. This study aimed to investigate the regulatory role of SPAG5 in CRC. The expression of SPAG5 in multiple cancers and normal tissues was predicted by The Cancer Genome Atlas and Tumor Immune Estimation Resource, and the expression of SPAG5 in human normal intestinal epithelial cells NCM460 and human CRC cell lines Caco2, HT29, SW480, and LOVO was verified by western blotting (WB). The effects of silencing SPAG5 on cell viability, proliferation, and apoptosis were then investigated by cell counting kit-8, WB, and flow cytometry. The effects of silencing SPAG5 on cell migration and invasion were investigated by scratch assay and transwell assay. Finally, the phosphorylation levels of phosphoinositide 3-kinase (PI3K) and AKT in cells were detected by WB. The results showed that SPAG5 was highly expressed in CRC and was verified by WB. Silencing of SPAG5 inhibited cell viability and proliferation and increased the cell apoptosis rate. Furthermore, both cell invasion and migration abilities were suppressed by the low expression of SPAG5. Finally, WB results found that the phosphorylation levels of PI3K and AKT were reduced after SPAG5 silencing. In summary, the results showed that SPAG5 can promote the proliferation and invasion of CRC cells by targeting the PI3K/AKT signaling pathway.
结直肠癌（CRC）是一种发生在直肠或结肠的癌症，发病率较高。精子相关抗原 5（SPAG5）是一种调节细胞分裂的基因，在多种癌症中观察到高度表达，但它在 CRC 中的作用尚不清楚。本研究旨在探讨 SPAG5 在 CRC 中的调控作用。通过癌症基因组图谱和肿瘤免疫估计资源预测 SPAG5 在多种癌症和正常组织中的表达，并通过 Western blot（WB）验证 SPAG5 在人正常肠上皮细胞 NCM460 和人 CRC 细胞系 Caco2、HT29、SW480 和 LOVO 中的表达。通过细胞计数试剂盒-8、WB 和流式细胞术研究沉默 SPAG5 对细胞活力、增殖和凋亡的影响。通过划痕试验和 Transwell 试验研究沉默 SPAG5 对细胞迁移和侵袭的影响。最后，通过 WB 检测细胞中磷酸肌醇 3-激酶（PI3K）和 AKT 的磷酸化水平。结果表明，SPAG5 在 CRC 中高表达，并通过 WB 验证。沉默 SPAG5 抑制细胞活力和增殖，增加细胞凋亡率。此外，低表达 SPAG5 抑制细胞侵袭和迁移能力。最后，WB 结果发现 SPAG5 沉默后 PI3K 和 AKT 的磷酸化水平降低。综上所述，结果表明 SPAG5 可通过靶向 PI3K/AKT 信号通路促进 CRC 细胞的增殖和侵袭。
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