Center for Vaccine Development and Global Health, University of Maryland School of Medicine, Baltimore, Maryland, USA.
US Army Institute of Surgical Research, Joint Base San Antonio Fort Sam Houston, Houston, Texas, USA.
mBio. 2023 Dec 19;14(6):e0245423. doi: 10.1128/mbio.02454-23. Epub 2023 Nov 6.
The interaction between an underlying disease process and a specific pathogen may lead to the unique expression of genes that affect bacterial pathogenesis. These genes may not be observed during infection in the absence of, or with a different underlying process or infection during the underlying process with a different pathogen. To test this hypothesis, we used Nanostring technology to compare gene transcription in a murine-burned wound infected with . The Nanostring probeset allowed the simultaneous direct comparison of immune response gene expression in both multiple host tissues and in conditions of burn alone, infection alone, and burn with infection. While RNA-Seq is used to discover novel transcripts, NanoString could be a technique to monitor specific changes in transcriptomes between samples and bypass the additional adjustments for multispecies sample processing or the need for the additional steps of alignment and assembly required for RNASeq. Using Nanostring, we identified arginine and IL-10 as important contributors to the lethal outcome of burned mice infected with . While other examples of altered gene transcription are in the literature, our study suggests that a more systematic comparison of gene expression in various underlying diseases during infection with specific bacterial pathogens may lead to the identification of unique host-pathogen interactions and result in more precise therapeutic interventions.
潜在疾病过程与特定病原体之间的相互作用可能导致影响细菌发病机制的特定基因的独特表达。这些基因在不存在或具有不同潜在过程的情况下，或在具有不同病原体的潜在过程中发生不同感染时，可能不会在感染期间观察到。为了验证这一假设，我们使用 Nanostring 技术比较了 感染的烧伤小鼠中的基因转录。Nanostring 探针集允许在单独烧伤、单独感染和烧伤合并感染的情况下，在多个宿主组织和 中同时直接比较免疫反应基因表达。虽然 RNA-Seq 用于发现新的转录本，但 NanoString 可以是一种监测样本之间转录组中特定变化的技术，而无需对多物种样本处理进行额外调整，也无需进行对齐和组装所需的额外步骤对于 RNASeq。使用 Nanostring，我们确定精氨酸和 IL-10 是 感染烧伤小鼠的致命结局的重要贡献者。虽然文献中有其他改变基因转录的例子，但我们的研究表明，在感染特定细菌病原体时，对各种潜在疾病中的基因表达进行更系统的比较，可能会导致发现独特的宿主-病原体相互作用，并导致更精确的治疗干预。
