Ji Yuxing, Pan Yanan, Ma Xuemei, Ma Yan, Zhao Zhongxiang, He Qiang
School of Pharmaceutical Sciences, Guangzhou University of Chinese Medicine, Guangzhou, 510006, China.
School of Medicine and Health, Harbin Institute of Technology, Harbin, 150001, China.
Chem Asian J. 2024 Jan 2;19(1):e202300879. doi: 10.1002/asia.202300879. Epub 2023 Nov 27.
We propose a glucose-powered Janus nanomotor where two faces are functionalized with glucose oxidase (GOx) and polydopamine-Fe chelates (PDF), respectively. In the glucose fuel solution, the GOx on the one side of these Janus nanomotors catalytically decomposes glucose fuels into gluconic acid and hydrogen peroxide (H O ) to drive them at a speed of 2.67 μm/s. The underlying propulsion mechanism is the glucose-based self-diffusiophoresis owing to the generated local glucose concentration gradient by the enzymatic reaction. Based on the enhanced diffusion motion, such nanomotors with catalytic activity increase the uptake towards cells and subsequently exhibit excellent capabilities for Fe ions delivery and H O generation for enhancing ferroptosis efficiency for inducing cancer cell death. In particular, the Fe ions are released from nanomotors in a slightly acidic environment, and subsequently generate toxic hydroxyl radicals via Fenton reactions, which accumulation reactive oxygen species (ROS) level (~300 %) and further lipid peroxidation (LPO) strengthened ferroptosis therapy for cancer treatment. The as-developed glucose-powered Janus nanomotor with efficient diffusion and Fe ions delivery capabilities show great promise as a potential in biomedical applications.
我们提出了一种以葡萄糖为动力的Janus纳米马达，其两个面分别用葡萄糖氧化酶（GOx）和聚多巴胺 - 铁螯合物（PDF）进行功能化修饰。在葡萄糖燃料溶液中，这些Janus纳米马达一侧的GOx将葡萄糖燃料催化分解为葡萄糖酸和过氧化氢（H₂O₂），使其以2.67 μm/s的速度驱动。其潜在的推进机制是基于酶促反应产生的局部葡萄糖浓度梯度的基于葡萄糖的自扩散电泳。基于增强的扩散运动，这种具有催化活性的纳米马达增加了对细胞的摄取，随后在铁离子递送和H₂O₂生成方面表现出优异的能力，以提高铁死亡效率从而诱导癌细胞死亡。特别地，铁离子在微酸性环境中从纳米马达释放，随后通过芬顿反应产生有毒的羟基自由基，这使活性氧（ROS）水平累积（约300 %）并进一步增强脂质过氧化（LPO），强化了用于癌症治疗的铁死亡疗法。所开发的具有高效扩散和铁离子递送能力的葡萄糖动力Janus纳米马达在生物医学应用中显示出作为一种潜力的巨大前景。
