Yang Feiying, Jing Xiaodong, Dong Renfu, Zhou Li, Xu Xuejiao, Dong Yuhong, Zhang Lingling, Zheng Ling, Lai Yingfang, Chen Yusong, Lin Lianyun, Ma Xiaoli, You Minsheng, Chen Wei, He Weiyi
State Key Laboratory for Ecological Pest Control of Fujian and Taiwan Crops, Institute of Applied Ecology, Fujian Agriculture and Forestry University, Fuzhou 350002, China.
Institute of Biological Resources, Jiangxi Academy of Sciences, Nanchang 330029, China.
J Agric Food Chem. 2023 Nov 6. doi: 10.1021/acs.jafc.3c06401.
Glucose oxidase (GOX) is a representative compound found in most insect saliva that can suppress plant-defensive responses. However, little is known about the origin and role of GOX in the crucifer-specialized pest . In this study, we showed obvious regurgitation from the larval gut of and identified abundant peptides highly similar to known GOX. Three genes were verified with preferentially expressed in the gut. The heterologously expressed PxGOX2 confirmed its function to be a GOX, and it was detected in plant wounds together with the gut regurgitant. Further experiments revealed that PxGOX2 functioned as an effector and may suppress defensive responses in plant through the production of HO, which modulates levels of antagonistic salicylic acid and jasmonic acid. However, excessive HO in the host plant may be neutralized by peroxidase, thus forming defensive feedback. Our findings provided new insights into understanding the GOX-mediated insect-plant interactions.
葡萄糖氧化酶（GOX）是大多数昆虫唾液中发现的一种代表性化合物，它可以抑制植物的防御反应。然而，关于GOX在十字花科特化害虫中的起源和作用知之甚少。在本研究中，我们观察到[具体昆虫名称]幼虫肠道有明显的反流现象，并鉴定出大量与已知GOX高度相似的肽段。验证了三个[昆虫名称]基因，其中[基因名称]在肠道中优先表达。异源表达的PxGOX2证实其功能为GOX，并且在植物伤口处与肠道反流物一起被检测到。进一步的实验表明，PxGOX2作为效应子发挥作用，可能通过产生H₂O₂来抑制植物的防御反应，H₂O₂可调节拮抗的水杨酸和茉莉酸水平。然而，宿主植物中过量的H₂O₂可能会被过氧化物酶中和，从而形成防御反馈。我们的研究结果为理解GOX介导的昆虫 - 植物相互作用提供了新的见解。
