Division of Molecular Microbiology, School of Life Sciences, University of Dundee, Dundee, UK.
Methods Mol Biol. 2024;2715:593-600. doi: 10.1007/978-1-0716-3445-5_37.
Antibacterial activity assays are an important tool in the assessment of the ability of one bacterium to kill or inhibit the growth of another, for example, during the study of the Type VI secretion system (T6SS) and the antibacterial toxins it secretes. The method we describe here can detect the ability of a bacterial strain to kill or inhibit other bacterial cells in a contact-dependent manner when cocultured on an agar surface. It is particularly useful since it enumerates the recovery of viable target cells and thus enables quantification of the antibacterial activity. We provide a detailed description of how to measure the T6SS-dependent antibacterial activity of a bacterium such as Serratia marcescens against a competitor prokaryotic organism, Escherichia coli, and describe possible variations in the method to allow adaptation to other attacker and target organisms.
抗菌活性测定是评估一种细菌杀死或抑制另一种细菌生长能力的重要工具，例如在研究 VI 型分泌系统（T6SS）及其分泌的抗菌毒素时。我们在这里描述的方法可以在琼脂表面共培养时检测细菌菌株以接触依赖性方式杀死或抑制其他细菌细胞的能力。由于它可以计算出目标细胞的存活恢复数，因此特别有用，从而能够定量测定抗菌活性。我们详细描述了如何测量细菌（如粘质沙雷氏菌）对竞争原核生物（大肠杆菌）的 T6SS 依赖性抗菌活性，并描述了该方法的可能变化，以适应其他攻击和目标生物。
