Alcoforado Diniz Juliana, Hollmann Birte, Coulthurst Sarah J
Division of Molecular Microbiology, School of Life Sciences, University of Dundee, Dow Street, Dundee, DD1 5EH, UK.
Methods Mol Biol. 2017;1615:517-524. doi: 10.1007/978-1-4939-7033-9_36.
Anti-bacterial activity assays are an important tool in the assessment of the ability of one bacterium to kill or inhibit the growth of another, for example during the study of the Type VI secretion system (T6SS) and the anti-bacterial toxins it secretes. The method we describe here can detect the ability of a bacterial strain to kill or inhibit other bacterial cells in a contact-dependent manner when co-cultured on an agar surface. It is particularly useful since it enumerates the recovery of viable target cells and thus enables quantification of the anti-bacterial activity. We provide a detailed description of how to measure the T6SS-dependent anti-bacterial activity of a bacterium such as Serratia marcescens against a competitor prokaryotic organism, Escherichia coli, and also describe possible variations in the method to allow adaptation to other attacker and target organisms.
抗菌活性测定是评估一种细菌杀死或抑制另一种细菌生长能力的重要工具，例如在研究VI型分泌系统（T6SS）及其分泌的抗菌毒素时。我们在此描述的方法能够检测在琼脂表面共培养时，一种细菌菌株以接触依赖方式杀死或抑制其他细菌细胞的能力。该方法特别有用，因为它能计数存活靶细胞的回收率，从而实现抗菌活性的量化。我们详细描述了如何测量诸如粘质沙雷氏菌等细菌对竞争原核生物大肠杆菌的T6SS依赖性抗菌活性，还描述了该方法可能的变体，以使其适用于其他攻击者和靶标生物。
