Tumor treating fields increases blood-brain barrier permeability and relative cerebral blood volume in patients with glioblastoma.

BACKGROUND AND OBJECTIVE

200 kHz tumor treating fields (TTFields) is clinically approved for newly-diagnosed glioblastoma (nGBM). Because its effects on conventional surveillance MRI brain scans are equivocal, we investigated its effects on perfusion MRI (pMRI) brain scans.

METHODS

Each patient underwent institutional standard pMRI: dynamic contrast-enhanced (DCE) and dynamic susceptibility contrast (DSC) pMRI at three time points: baseline, 2-, and 6-months on-adjuvant therapy. At each timepoint, the difference between T1 pre- versus post-contrast tumor volume (ΔT1) and these pMRI metrics were evaluated: normalized and standardized relative cerebral blood volume (nRCBV, sRCBV); fractional plasma volume (V), volume of extravascular extracellular space (EES) per volume of tissue (V), blood-brain barrier (BBB) permeability (K), and time constant for gadolinium reflux from EES back into the vascular system (K). Between-group comparisons were performed using rank-sum analysis, and bootstrapping evaluated likely reproducibility of the results.

RESULTS

Among 13 pMRI datasets (11 nGBM, 2 recurrent GBM), therapies included temozolomide-only ( = 9) and temozolomide + TTFields ( = 4). No significant differences were found in patient or tumor characteristics. Compared to temozolomide-only, temozolomide + TTFields did not significantly affect the percent-change in pMRI metrics from baseline to 2 months. But during the 2- to 6-month period, temozolomide + TTFields significantly increased the percent-change in nRCBV (+26.9% [interquartile range 55.1%] vs -39.1% [37.0%], = 0.049), sRCBV (+9.5% [39.7%] vs -30.5% [39.4%], = 0.049), K (+54.6% [1768.4%] vs -26.9% [61.2%], = 0.024), V (+111.0% [518.1%] vs -13.0% [22.5%], = 0.048), and V (+98.8% [2172.4%] vs -24.6% [53.3%], = 0.024) compared to temozolomide-only.

CONCLUSION

Using pMRI, we provide initial in-human validation of pre-clinical studies regarding the effects of TTFields on tumor blood volume and BBB permeability in GBM.