Sagai T, Shiroishi T, Moriwaki K, Bonhomme F, Petras M L, Thohari M, Yu Z C, Lu D Y, Cho W S
Immunogenetics. 1986;24(6):361-7. doi: 10.1007/BF00377953.
We have already developed nine B10.MOL congenic strains carrying H-2 haplotypes derived from Japanese wild mice, Mus musculus molossinus, with the C57BL/10 genetic background. To obtain monoclonal antibodies against the H-2 antigen of the Japanese wild mouse, we carried out cell fusion using spleen cells from the animal immunized with one of the B10.MOL strains, B10.MOL-SGR (H-2wm7). As a result, 19 hybridomas producing monoclonal antibodies were produced. Analysis with the intra-H-2 recombinants derived from B10.MOL-SGR indicated that 8 of them reacted with the class I and 11 with the class II molecule. The class I antibodies were tested for their cross-reactivities on wild mice and on the panels of standard inbred and B10.MOL strains. Most of the antibodies reacted with both the Japanese wild mice and the other subspecies, including standard inbred, while two antibodies highly specific for the donor H-2K region reacted with only three wild-derived mice, two M. m. molossinus from Anjo and Shizuoka, Japan, and one M. m. domesticus from Pigeon, Canada. In addition, all of the other four antibodies reactive with the K antigen of B10.MOL-SGR also reacted with the same three wild mice. The wild mice belonging to different subspecies might share very similar H-2K antigenic determinants in spite of their genetic and geographical remoteness.
我们已经培育出了9种B10.MOL同源近交系，其携带源自日本野生小鼠（小家鼠）的H-2单倍型，具有C57BL/10的遗传背景。为了获得针对日本野生小鼠H-2抗原的单克隆抗体，我们使用了用其中一种B10.MOL品系B10.MOL-SGR（H-2wm7）免疫的动物的脾细胞进行细胞融合。结果，产生了19个分泌单克隆抗体的杂交瘤。对源自B10.MOL-SGR的H-2内部重组体的分析表明，其中8个与I类分子反应，11个与II类分子反应。对I类抗体在野生小鼠以及标准近交系和B10.MOL品系的面板上的交叉反应性进行了测试。大多数抗体与日本野生小鼠以及其他亚种反应，包括标准近交系，而两种对供体H-2K区域高度特异性的抗体仅与三只野生来源的小鼠反应，两只来自日本安城和静冈的小家鼠，以及一只来自加拿大鸽子的小家鼠。此外，与B10.MOL-SGR的K抗原反应的其他四种抗体也与相同的三只野生小鼠反应。尽管不同亚种的野生小鼠在遗传和地理上相距遥远，但它们可能共享非常相似的H-2K抗原决定簇。
