小鼠主要组织相容性复合体III类区域的重组

Recombination in the class III region of the mouse major histocompatibility complex.

作者信息

Yoshino M, Sagai T, Lindahl K F, Toyoda Y, Shirayoshi Y, Matsumoto K, Sugaya K, Ikemura T, Moriwaki K, Shiroishi T

机构信息

Mammalian Genetics Laboratory, National Institute of Genetics, Mishima, Japan.

出版信息

Immunogenetics. 1994;40(4):280-6. doi: 10.1007/BF00189973.

DOI:10.1007/BF00189973

PMID:7916002

Abstract

The sites of meiotic recombination in the class II region of the mouse major histocompatibility complex (MHC) are clustered at hotspots. To search for hotspots in the class III region, we mapped recombinational breakpoints of 79 Ab:H2-D recombinants with 11 DNA markers; these included Tnx, the gene for an extracellular matrix protein, tenascin X, the Notch-related Int3 gene, and a microsatellite marker, D17Mit13, none of which had previously been mapped precisely. The results gave the gene order of Eb-61.1-Int3-Tnx-Cyp21/C4-Bf-Hsp68c-D17Mit13+ ++-Tnfa/Tnfb-D. The crossover sites in 40 of the 79 recombinants were confined within the Eb/Int3:Tnx/Cyp21 interval. The result demonstrated that an unequal distribution of recombination is a general feature of the mouse MHC, suggesting the presence of a recombinational hotspot within the Int3:Tnx interval.

摘要

小鼠主要组织相容性复合体（MHC）II类区域减数分裂重组的位点聚集在热点区域。为了在III类区域寻找热点，我们用11个DNA标记绘制了79个Ab:H2-D重组体的重组断点图；这些标记包括细胞外基质蛋白腱生蛋白X的基因Tnx、与Notch相关的Int3基因以及一个微卫星标记D17Mit13，之前它们都没有被精确绘制过图谱。结果得出基因顺序为Eb-61.1-Int3-Tnx-Cyp21/C4-Bf-Hsp68c-D17Mit13++++-Tnfa/Tnfb-D。79个重组体中有40个的交叉位点局限在Eb/Int3:Tnx/Cyp21区间内。该结果表明重组的不均衡分布是小鼠MHC的一个普遍特征，提示在Int3:Tnx区间内存在一个重组热点。