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BS 感染患者粪便微生物群和循环 microRNA-16 特征分析：病例对照研究。

Profiling of the fecal microbiota and circulating microRNA-16 in IBS subjects with Blastocystis infection : a case-control study.

机构信息

Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran.

Foodborne and Waterborne Diseases Research Center, Research Institute for Gastroenterology and Liver Diseases, Shahid Beheshti University of Medical Sciences, Tehran, Iran.

出版信息

Eur J Med Res. 2023 Nov 6;28(1):483. doi: 10.1186/s40001-023-01441-8.

DOI:10.1186/s40001-023-01441-8
PMID:37932792
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10626746/
Abstract

Irritable bowel syndrome (IBS) is a prevalent gastrointestinal (GI) tract disorder. Although the main reason for IBS is not clear, the interaction between intestinal microorganisms and the gut barrier seems to play an important role in pathogenesis of IBS. The current study aimed to investigate the effect of Blastocystis on the gut microbiota profile and the circulation levels of microRNA (mir)-16 of IBS patients compared to healthy subjects. Stool and blood samples were collected from 80 participants including 40 samples from each IBS and healthy group. Upon DNA extraction from stool samples, barcoding region and quantitative real-time PCR were analyzed to investigate Blastocystis and the microbiota profile, respectively. RNA was extracted from serum samples of included subjects and the expression of mir-16 was evaluated using stem-loop protocol and qreal-time PCR. Significant changes between IBS patients and healthy controls was observed in Firmicutes, Actinobacteria, Faecalibacterium, and Alistipes. In IBS patients, the relative abundance of Bifidobacteria was directly correlated with the presence of Blastocystis, while Alistipes was decreased with Blastocystis. Lactobacillus was significantly increased in Blastocystis carriers. In healthy subjects, the relative abundance of Bifidobacteria was decreased, but Alistipes was increased in Blastocystis carriers. The changes in the Firmicutes/Bacteroidetes ratio was not significant in different groups. The relative expression of mir-16 in Blastocystis-negative IBS patients and healthy carriers was significantly overexpressed compared to control group. The presence of Blastocystis, decreased the relative expression of mir-16 in IBS patients compared to Blastocystis-negative IBS patients. The present study revealed that Blastocystis has the ability to change the abundance of some phyla/genera of bacteria in IBS and healthy subjects. Moreover, Blastocystis seems to  modulate the relative expression of microRNAs  to control the gut atmosphere, apply its pathogenicity, and provide a favor niche for its colonization.

摘要

肠易激综合征（IBS）是一种常见的胃肠道（GI）疾病。虽然 IBS 的主要原因尚不清楚，但肠道微生物和肠道屏障之间的相互作用似乎在 IBS 的发病机制中起重要作用。本研究旨在比较 IBS 患者和健康受试者粪便微生物群谱和循环 microRNA（mir）-16 水平的差异。从 80 名参与者中收集粪便和血液样本，其中 IBS 组和健康组各 40 例。从粪便样本中提取 DNA 后，通过条形码区域和定量实时 PCR 分析来分别研究 Blastocystis 和微生物群谱。从纳入研究对象的血清样本中提取 RNA，并使用茎环法和 qreal-time PCR 评估 mir-16 的表达。在 IBS 患者和健康对照组之间观察到厚壁菌门、放线菌门、粪杆菌和alistipes 的显著变化。在 IBS 患者中，双歧杆菌的相对丰度与 Blastocystis 的存在呈直接相关，而 alistipes 则随着 Blastocystis 的存在而减少。双歧杆菌在 Blastocystis 携带者中显著增加。在健康受试者中，双歧杆菌的相对丰度降低，但 Blastocystis 携带者的 alistipes 增加。不同组之间厚壁菌门/拟杆菌门比值的变化不显著。与对照组相比，Blastocystis 阴性 IBS 患者和健康携带者的 mir-16 相对表达显著上调。与 Blastocystis 阴性 IBS 患者相比，Blastocystis 的存在降低了 IBS 患者 mir-16 的相对表达。本研究表明，Blastocystis 能够改变 IBS 和健康受试者中一些菌门/属的丰度。此外，Blastocystis 似乎可以调节 microRNAs 的相对表达，以控制肠道环境，发挥其致病性，并为其定植提供有利的生态位。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9374/10626746/7941bd332572/40001_2023_1441_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9374/10626746/ffce93e5f1a8/40001_2023_1441_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9374/10626746/96e947daf319/40001_2023_1441_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9374/10626746/2e64fca477b2/40001_2023_1441_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9374/10626746/b395dfae0fe0/40001_2023_1441_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9374/10626746/7941bd332572/40001_2023_1441_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9374/10626746/ffce93e5f1a8/40001_2023_1441_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9374/10626746/96e947daf319/40001_2023_1441_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9374/10626746/2e64fca477b2/40001_2023_1441_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9374/10626746/b395dfae0fe0/40001_2023_1441_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9374/10626746/7941bd332572/40001_2023_1441_Fig5_HTML.jpg

相似文献

[1]
Profiling of the fecal microbiota and circulating microRNA-16 in IBS subjects with Blastocystis infection : a case-control study.

Eur J Med Res. 2023-11-6

[2]
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[3]
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[6]
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[7]
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[8]
A starch- and sucrose-reduced dietary intervention in irritable bowel syndrome patients produced a shift in gut microbiota composition along with changes in phylum, genus, and amplicon sequence variant abundances, without affecting the micro-RNA levels.

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[9]
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[10]
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引用本文的文献

[1]
Systematic review of bidirectional interaction between gut microbiome, miRNAs, and human pathologies.

Front Microbiol. 2025-2-5

[2]
Chronic Gastrointestinal Disorders and miRNA-Associated Disease: An Up-to-Date.

Int J Mol Sci. 2025-1-6

[3]
Global research states and trends of micro RNA in irritable bowel syndrome: a bibliometric analysis.

Clin Exp Med. 2024-7-5

本文引用的文献

[1]
Intestinal microbiota changes pre- and post-fecal microbiota transplantation for treatment of recurrent infection among Iranian patients with concurrent inflammatory bowel disease.

Front Microbiol. 2023-2-24

[2]
Colonization with two different subtypes in DSS-induced colitis mice is associated with strikingly different microbiome and pathological features.

Theranostics. 2023

[3]
Alternation of the gut microbiota in irritable bowel syndrome: an integrated analysis based on multicenter amplicon sequencing data.

J Transl Med. 2023-2-11

[4]
MicroRNAs: Key modulators of inflammation-associated diseases.

Semin Cell Dev Biol. 2024-2-15

[5]
The Role of Short Chain Fatty Acids in Irritable Bowel Syndrome.

J Neurogastroenterol Motil. 2022-10-30

[6]
MicroRNA-16 inhibits the TLR4/NF-κB pathway and maintains tight junction integrity in irritable bowel syndrome with diarrhea.

J Biol Chem. 2022-11

[7]
Exosomes secreted by subtypes affect the expression of proinflammatory and anti-inflammatory cytokines (TNFα, IL-6, IL-10, IL-4).

Front Med (Lausanne). 2022-8-11

[8]
Gut Microbiota Shifting in Irritable Bowel Syndrome: The Mysterious Role of sp.

Front Med (Lausanne). 2022-6-20

[9]
Presence of Blastocystis in gut microbiota is associated with cognitive traits and decreased executive function.

ISME J. 2022-9

[10]
Serological levels of cytokines in irritable bowel syndrome (IBS) patients and non-IBS subjects with and without Blastocystis spp. infection.

Ann Parasitol. 2022

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