伞形花内酯标记的半乳糖胺作为鉴定白色念珠菌的辅助手段。
Umbelliferyl-labeled galactosaminide as an aid in identification of Candida albicans.
作者信息
Perry J L, Miller G R
机构信息
Clinical Microbiology Laboratories, Veterans Administration Medical Center, Wichita, Kansas 67211.
出版信息
J Clin Microbiol. 1987 Dec;25(12):2424-5. doi: 10.1128/jcm.25.12.2424-2425.1987.
Abstract
The initial evaluation of a fluorescence, nonmicroscopic method of rapid identification of Candida albicans is described. A total of 524 yeast isolates were evaluated in parallel by the umbelliferyl-conjugated N-acetyl-beta-D-galactosaminide (UAG) test and the germ tube (GT) test in comparison with the API 20C Yeast Identification System. The UAG test correctly identified 333 of the 334 isolates of C. albicans (99.7%), and the GT test identified 328 (98%). There were three false-positive GT reactions and five false-positive UAG reactions with 69 isolates of C. tropicalis. The sensitivity and specificity were 99 and 97%, respectively, for the UAG test and were both 98% for the GT test. The UAG test requires less than 2 h for test completion compared with 2 to 4 h for the GT test, is similar in cost, has a nonmicroscopic visual endpoint, and eliminates the health hazards of handling pooled human sera.
摘要
本文描述了一种用于快速鉴定白色念珠菌的荧光非显微镜方法的初步评估。通过伞形基共轭N-乙酰-β-D-半乳糖胺（UAG）试验和芽管（GT）试验，与API 20C酵母鉴定系统并行评估了总共524株酵母分离株。UAG试验正确鉴定出334株白色念珠菌中的333株（99.7%），GT试验鉴定出328株（98%）。对于69株热带念珠菌，有3例假阳性GT反应和5例假阳性UAG反应。UAG试验的敏感性和特异性分别为99%和97%，GT试验的敏感性和特异性均为98%。与GT试验需要2至4小时相比，UAG试验完成测试所需时间不到2小时，成本相似，具有非显微镜视觉终点，并且消除了处理混合人血清的健康危害。
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