Raaphorst G P, Vadasz J, Azzam E I
Int J Radiat Oncol Biol Phys. 1986 Dec;12(12):2151-5. doi: 10.1016/0360-3016(86)90014-3.
The mouse C3H 10T1/2 cell line was transformed to the malignant state using ionizing radiation. One of the transformed lines (R25) that was isolated, displayed some properties similar to malignant melanoma cells. The cells became dark and pigmented after prolonged time in culture and this cell line produced tumors in C3H mice. The radiation survival curve of R25 had a large shoulder which was also observed for human melanoma cell lines. R25 was more resistant to heating at 45.0 degrees C than the normal cell line. Heating at 45.0 degrees C before irradiation resulted in a reduction of the survival curve shoulder. The heat and radiation sensitivity of R25 did not appear to be related to the melanin content of these cells.
利用电离辐射将小鼠C3H 10T1/2细胞系转化为恶性状态。分离得到的其中一个转化细胞系（R25）表现出一些与恶性黑色素瘤细胞相似的特性。培养较长时间后，细胞变黑并产生色素，该细胞系在C3H小鼠体内产生肿瘤。R25的辐射存活曲线有一个很大的肩区，人类黑色素瘤细胞系也观察到这一现象。R25比正常细胞系对45.0℃加热更具抗性。照射前在45.0℃加热会导致存活曲线肩区减小。R25的热敏感性和辐射敏感性似乎与这些细胞的黑色素含量无关。
