Raaphorst G P, Azzam E I
Medical Physics Department, Ottawa Regional Cancer Centre, Ontario, Canada.
Int J Hyperthermia. 1988 May-Jun;4(3):345-54. doi: 10.3109/02656738809051109.
C3H-10T1/2 cells were induced to a thermotolerant state by heating at 41.0 degrees C, 42.0 degrees C, 45.0 degrees C or by treatment with ethanol. As thermotolerance developed, cellular heat sensitivity and thermoradiosensitization decreased. Transformation was measured by scoring transformed foci as previously described (Raaphorst et al. 1986 a). Hyperthermia alone in normal and thermotolerant cells did not increase the transformation frequency above background levels. The level of transformation induced by irradiation increased up to three-fold as cells became thermotolerant. This was observed for thermotolerance induced by long-term heating at 41.0 degrees C or short acute heat treatments at 42.0 degrees C or 45.0 degrees C followed by incubation at 37.0 degrees C. Treatment with ethanol also induced thermotolerance, and reduced radiosensitization and increased transformation in the thermotolerant state.
将C3H - 10T1/2细胞在41.0℃、42.0℃、45.0℃加热或用乙醇处理，诱导其进入耐热状态。随着耐热性的发展，细胞的热敏感性和热辐射敏感性降低。如前所述（Raaphorst等人，1986年a），通过对转化灶进行评分来测量转化。单独的热疗在正常细胞和耐热细胞中并不会使转化频率增加到背景水平以上。随着细胞变得耐热，辐射诱导的转化水平增加了两倍。在通过41.0℃长期加热或42.0℃或45.0℃短时间急性热处理后于37.0℃孵育诱导的耐热性中观察到了这种情况。用乙醇处理也诱导了耐热性，并降低了辐射敏感性，且在耐热状态下增加了转化。
