Fibach E, Morand O, Gatt S
J Cell Sci. 1986 Sep;85:149-59. doi: 10.1242/jcs.85.1.149.
Cells were incubated with 12-(1-pyrene)-dodecanoic acid (P12), a long-chain fatty acid to which a pyrene ring has been attached covalently. This acid was transported across the plasma membranes of cells and subsequently incorporated into their neutral lipids and phospholipids. Irradiation of these pyrene-containing cells for short periods (0.5-4 min) with ultraviolet light at 366 nm resulted in eventual cell death. Similar irradiation had no effect on cells that had not been exposed to P12. The time of the period of irradiation necessary for inducing the toxic process was related to the quantity of P12 incorporated, the latter being a function of the respective metabolic activity of the individual cell type. The degree of incorporation of P12 into a cell, and consequently its acquired sensitivity to killing by ultraviolet irradiation at 366 nm, was affected by the incubation temperature and addition of non-fluorescent fatty acid, albumin or other serum proteins. Different degrees of incorporation of P12 into various cell types were used for selective killing and elimination of cell populations by irradiation at 366 nm. The combined procedure of preincubation with P12 followed by ultraviolet irradiation thus permitted selection of cell types with a greater resistance to this procedure.
细胞与12-(1-芘基)-十二烷酸（P12）一起孵育，P12是一种共价连接了芘环的长链脂肪酸。这种酸被转运穿过细胞膜，随后掺入细胞的中性脂质和磷脂中。用366nm的紫外线对这些含芘细胞进行短时间（0.5 - 4分钟）照射会导致细胞最终死亡。类似的照射对未接触P12的细胞没有影响。诱导毒性过程所需的照射时间与掺入的P12量有关，而P12量是各个细胞类型各自代谢活性的函数。P12掺入细胞的程度，以及因此其对366nm紫外线杀伤所获得的敏感性，受孵育温度以及非荧光脂肪酸、白蛋白或其他血清蛋白添加的影响。通过366nm照射利用P12在各种细胞类型中不同程度的掺入来选择性杀伤和清除细胞群体。先与P12预孵育然后进行紫外线照射的联合程序因此允许选择对该程序具有更大抗性的细胞类型。
