Centro Andaluz de Biología del Desarrollo, CSIC, Universidad Pablo de Olavide, Sevilla, Spain.
Instituto de Biotecnología, Universidad Nacional Autónoma de México, Cuernavaca, Morelos, México.
Nat Commun. 2023 Nov 9;14(1):7224. doi: 10.1038/s41467-023-43096-3.
Planctopirus limnophila belongs to the bacterial phylum Planctomycetes, a relatively understudied lineage with remarkable cell biology features. Here, we report a genome-wide analysis of essential gene content in P. limnophila. We show that certain genes involved in peptidoglycan synthesis or cell division, which are essential in most other studied bacteria, are not essential for growth under laboratory conditions in this species. We identify essential genes likely involved in lipopolysaccharide biosynthesis, consistent with the view of Planctomycetes as diderm bacteria, and highlight other essential genes of unknown functions. Furthermore, we explore potential stages of evolution of the essential gene repertoire in Planctomycetes and the related phyla Verrucomicrobia and Chlamydiae. Our results provide insights into the divergent molecular and cellular biology of Planctomycetes.
滨螺菌属于相对研究较少的浮霉状菌门，具有显著的细胞生物学特征。在这里，我们报告了滨螺菌全基因组必需基因内容的分析。我们表明，某些参与肽聚糖合成或细胞分裂的基因，在大多数其他研究的细菌中是必需的，但在该物种的实验室条件下生长并非必需。我们鉴定了可能参与脂多糖生物合成的必需基因，这与浮霉状菌作为双壁菌的观点一致，并强调了其他未知功能的必需基因。此外，我们还探讨了浮霉状菌及其相关的疣微菌门和衣原体门的必需基因库的潜在进化阶段。我们的研究结果为浮霉状菌的不同分子和细胞生物学提供了新的见解。
