Henriques Valentina, Moyano-Palazuelo David, Alonso-Pascual Maria Teresa, Pazos Manuel, Devos Damien P, Rivas-Marin Elena
Centro Andaluz de Biología del Desarrollo-CSIC, Pablo de Olavide University, Seville, Spain.
Laboratory of Mass Spectrometry (SIdI-UAM), Autonomous University of Madrid, Madrid, Spain.
iScience. 2025 Jun 30;28(8):113037. doi: 10.1016/j.isci.2025.113037. eCollection 2025 Aug 15.
The -- superphylum comprises bacteria with divergent traits, epitomizing the vast biodiversity of non-model organisms that remains unexplored. Among its members, and exhibit unique adaptations, including variations in peptidoglycan structure and cell division without FtsZ. represents the only name-giving phylum within the PVC not extensively investigated. Here, exhaustive transposon mutagenesis combined with massive sequencing reveal the essential genome of . The essentiality report demonstrates that the cluster is essential except . Additionally, peptidoglycan analysis evidences that the composition and saccular arrangement are canonical, contrarily to and Targeted mutagenesis for was also developed, thus reinforcing its status as a model for . Ultimately, the evolutionary divergences of the cluster in PVC, combined with the previous results, suggest that its Last Common Ancestor was a Gracilicutes-like bacterium dividing by an FtsZ-mediated binary fission with peptidoglycan, from which current phyla diverged.
这个超门包含具有不同特征的细菌，是尚未被探索的非模式生物巨大生物多样性的典型代表。在其成员中，[具体名称1]和[具体名称2]表现出独特的适应性，包括肽聚糖结构的变化和无FtsZ的细胞分裂。[具体名称3]是PVC内唯一未被广泛研究的有命名的门。在这里，详尽的转座子诱变结合大规模测序揭示了[具体名称3]的必需基因组。必需性报告表明，除了[具体情况]外，[具体名称3]的[具体基因簇]是必需的。此外，肽聚糖分析证明其组成和囊状排列是典型的，这与[具体名称1]和[具体名称2]相反。还开发了针对[具体名称3]的靶向诱变方法，从而加强了其作为[具体研究对象]模型的地位。最终，PVC中[具体基因簇]的进化分歧，结合先前的结果，表明其最后的共同祖先是一种类似薄壁菌门的细菌，通过FtsZ介导的二元裂变与肽聚糖进行分裂，当前的各个门由此分化而来。
